Polypeptide from Moschus Suppresses Lipopolysaccharide-Induced Inflammation by Inhibiting NF-κ B-ROS/NLRP3 Pathway

Objective To examine the anti-inflammatory effects and potential mechanisms of polypeptide from Moschus (PPM) in lipopolysaccharide (LPS)-induced THP-1 macrophages and BALB/c mice. Methods The polypeptide was extracted from Moschus and analyzed by high-performance liquid chromatography and sodium do...

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Published in:Chinese journal of integrative medicine 2023-10, Vol.29 (10), p.895-904
Main Authors: Yi, Jing, Li, Li, Yin, Zhu-jun, Quan, Yun-yun, Tan, Rui-rong, Chen, Shi-long, Lang, Ji-rui, Li, Jiao, Zeng, Jin, Li, Yong, Sun, Zi-jian, Zhao, Jun-ning
Format: Article
Language:English
Subjects:
Medicine
Medicine & Public Health
Original Article
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Summary:Objective To examine the anti-inflammatory effects and potential mechanisms of polypeptide from Moschus (PPM) in lipopolysaccharide (LPS)-induced THP-1 macrophages and BALB/c mice. Methods The polypeptide was extracted from Moschus and analyzed by high-performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Subsequently, LPS was used to induce inflammation in THP-1 macrophages and BALB/c mice. In LPS-treated or untreated THP-1 macrophages, cell viability was observed by cell counting kit 8 and lactate dehydrogenase release assays; the proinflammatory cytokines and reactive oxygen species (ROS) were measured by enzyme-linked immunosorbent assay and flow cytometry, respectively; and protein and mRNA levels were measured by Western blot and real-time quantitative polymerase chain reaction (qRT-PCR), respectively. In LPS-induced BALB/c mice, the proinflammatory cytokines were measured, and lung histology and cytokines were observed by hematoxylin and eosin (HE) and immunohistochemical (IHC) staining, respectively. Results The SDS-PAGE results suggested that the molecular weight of purified PPM was in the range of 10–26 kD. In vitro , PPM reduced the production of interleukin 1β (IL-1β), IL-18, tumor necrosis factor α (TNF-α), IL-6 and ROS in LPS-induced THP-1 macrophages ( P
ISSN:1672-0415
1993-0402
DOI:10.1007/s11655-023-3598-z