A kinetic characterization of the gill V(H+)-ATPase from two hololimnetic populations of the Amazon River shrimp Macrobrachium amazonicum

This investigation examines the kinetic characteristics and effect of acclimation to a brackish medium (21 ‰S) on gill V(H+)-ATPase activity in two hololimnetic populations of M. amazonicum. We also investigate the cellular immunolocalization of the enzyme. Immunofluorescence findings demonstrate th...

Full description

Saved in:
Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2023-10, Vol.268, p.110880-110880, Article 110880
Main Authors: Fabri, Leonardo M., Garçon, Daniela P., Moraes, Cintya M., Pinto, Marcelo R., McNamara, John C., Leone, Francisco A.
Format: Article
Language:English
Subjects:
Bafilomycin
Freshwater shrimp
Macrobrachium amazonicum
Orthovanadate
Salinity acclimation
V(H+)-ATPase kinetics
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This investigation examines the kinetic characteristics and effect of acclimation to a brackish medium (21 ‰S) on gill V(H+)-ATPase activity in two hololimnetic populations of M. amazonicum. We also investigate the cellular immunolocalization of the enzyme. Immunofluorescence findings demonstrate that the V(H+)-ATPase c-subunit is distributed in the apical pillar cells of shrimps in fresh water but is absent after acclimation to 21 ‰S for 10 days. V(H+)-ATPase activity from the Tietê River population is ≈50% greater than the Grande River population, comparable to a wild population from the Santa Elisa Reservoir, but is 2-fold less than in cultivated shrimps. V(H+)-ATPase activity in the Tietê and the Grande River shrimps is abolished after 21 ‰S acclimation. The apparent affinities of the V(H+)-ATPase for ATP (0.27 ± 0.04 and 0.16 ± 0.03 mmol L−1, respectively) and Mg2+ (0.28 ± 0.05 and 0.14 ± 0.02 mmol L−1, respectively) are similar in both populations. The absence of V(H+)-ATPase activity in salinity-acclimated shrimps and its apical distribution in shrimps in fresh water underpins the importance of the crustacean V(H+)-ATPase for ion uptake in fresh water. [Display omitted] •V(H+)-ATPase activity is ≈60% greater in the Tietê than Grande River shrimp population.•ATP hydrolysis by the V(H+)-ATPase follows Michaelian kinetics in both populations.•25% of V(H+)-ATPase activity occurs in sealed microsomes in Grande River shrimps.•In fresh water, the V(H+)-ATPase is immunolocalized to the apical pillar cell flanges.
ISSN:1096-4959
1879-1107
DOI:10.1016/j.cbpb.2023.110880