Unraveling the role of effector proteins in Bipolaris oryzae infecting North East Indian rice cultivars through time-course transcriptomics analysis

Bipolaris oryzae, causing brown spot disease in rice, is one of the neglected diseases reducing rice productivity. Limited knowledge is available on the genetics of host-pathogen interaction. Here, we used time-course transcriptome sequencing to elucidate the differential transcriptional responses o...

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Bibliographic Details
Published in:Fungal biology 2023-07, Vol.127 (7-8), p.1098-1110
Main Authors: Singh, Sanjay, Sarki, Yogita N., Marwein, Riwandahun, Singha, Dhanawantari L., Velmurugan, Natarajan, Chikkaputtaiah, Channakeshavaiah
Format: Article
Language:English
Subjects:
Brown spot disease
CAZyme
Effector
Pathogen
Rice
Sequencing
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Summary:Bipolaris oryzae, causing brown spot disease in rice, is one of the neglected diseases reducing rice productivity. Limited knowledge is available on the genetics of host-pathogen interaction. Here, we used time-course transcriptome sequencing to elucidate the differential transcriptional responses of the pathogen genes in two contradictory infection-responsive rice hosts. Evaluation of transcriptome data showed similar regulation of fungal genes within susceptible (1733) and resistant (1846) hosts at an early stage however, in the later stage, the number was significantly higher in susceptible (2877) compared to resistant (1955) hosts. GO enrichment terms for upregulated genes showed a similar pattern in both the hosts at an early stage, but in the later stage terms related to degradation of carbohydrates, carbohydrate transport, and pathogenesis are enriched extensively within the susceptible host. Likewise, similar expression responses were observed with the secretory and effector proteins. Plant pathogenic homologs genes such as those involved in appressorium and conidia formation, host cell wall degradative enzymes, etc. were reported to be highly upregulated within the susceptible host. This study predicts the successful establishment of B. oryzae BO1 in both the host surfaces at an early stage, while disease progression only occurs in the susceptible host in later stage. •Bipolaris oryzae exhibited differential gene responses at a later stage than the early stage of infection.•The differential response of pathogen was shaped by host immunity.•Effector proteins and CAZymes were major modulators at a later stage of infection.•First dataset of B. oryzae transcriptional responses in two contrasting rice hosts.
ISSN:1878-6146
1878-6162
DOI:10.1016/j.funbio.2023.05.003