In vivo evaluation of bone regeneration using ZIF8-modified polypropylene membrane in rat calvarium defects

The profound potential of zeolitic imidazolate framework 8 (ZIF8) thin film for inducing osteogenesis has been previously established under in vitro conditions. As the next step towards the clinical application of ZIF8-modified substrates in periodontology, this in vivo study aimed to evaluate the a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical periodontology 2023-10, Vol.50 (10), p.1390-1405
Main Authors: Mousavi, Seyed Javad, Ejeian, Fatemeh, Razmjou, Amir, Nasr-Esfahani, Mohammad Hossein
Format: Article
Language:English
Subjects:
Animal models
Bone growth
Bone surgery
Coatings
Computed tomography
Mechanical properties
Osteogenesis
Polypropylene
Regeneration
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The profound potential of zeolitic imidazolate framework 8 (ZIF8) thin film for inducing osteogenesis has been previously established under in vitro conditions. As the next step towards the clinical application of ZIF8-modified substrates in periodontology, this in vivo study aimed to evaluate the ability of the ZIF8 crystalline layer to induce bone regeneration in an animal model defect. Following the mechanical characterization of the membranes and analysing the in vitro degradation of the ZIF8 layer, in vivo bone regeneration was evaluated in a critical-sized (5-mm) rat calvarial bone defect model. For each animal, one defect was randomly covered with either a polypropylene (PP) or a ZIF8-modified membrane (n = 7 per group), while the other defect was left untreated as a control. Eight weeks post surgery, bone formation was assessed by microcomputed tomography scanning, haematoxylin and eosin staining and immunohistochemical analysis. The ZIF8-modified membrane outperformed the PP membrane in terms of mechanical properties and revealed a trace Zn release. Results of in vivo evaluation verified the superior barrier function of the ZIF8-coated membrane compared with pristine PP membrane. Compared with the limited marginal bone formation in the control and PP groups, the defect area was almost filled with mature bone in the ZIF8-coated membrane group. Our results support the effectiveness of the ZIF8-coated membrane as a promising material for improving clinical outcomes of guided bone regeneration procedures, without using biological components.
ISSN:0303-6979
1600-051X
1600-051X
DOI:10.1111/jcpe.13855