Leukocyte‐poor platelet‐rich plasma and leukocyte‐rich platelet‐rich plasma promote myoblast proliferation through the upregulation of cyclin A, cdk1, and cdk2

Muscle injuries are common among athletes and often treated with platelet‐rich plasma (PRP). However, whether the leukocyte concentration affects the efficacy of PRP in treating muscle injuries remains unclear. This study investigated the effects of leukocyte‐poor platelet‐rich plasma (LP‐PRP) and l...

Full description

Saved in:
Bibliographic Details
Published in:Journal of orthopaedic research 2024-01, Vol.42 (1), p.32-42
Main Authors: Chen, Li‐Siou, Chen, Chih‐Kuang, Pang, Jong‐Hwei Su, Lin, Li‐Ping, Yu, Tung‐Yang, Tsai, Wen‐Chung
Format: Article
Language:English
Subjects:
CDC2 Protein Kinase - metabolism
Cell Proliferation
Cyclin A - metabolism
Humans
leukocyte
Leukocytes - physiology
myoblast
Myoblasts - physiology
Platelet-Rich Plasma - metabolism
platelet‐rich plasma
proliferation
Up-Regulation
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Muscle injuries are common among athletes and often treated with platelet‐rich plasma (PRP). However, whether the leukocyte concentration affects the efficacy of PRP in treating muscle injuries remains unclear. This study investigated the effects of leukocyte‐poor platelet‐rich plasma (LP‐PRP) and leukocyte‐rich platelet‐rich plasma (LR‐PRP) on myoblast proliferation and the molecular mechanisms underlying these effects. Myoblasts were treated with 0.5% LP‐PRP, 0.5% LR‐PRP, 1% LP‐PRP, or 1% LR‐PRP for 24 h. The gene expression of the LP‐PRP‐ and LR‐PRP–treated myoblasts was determined using RNA sequencing analysis. Cell proliferation was evaluated using an bromodeoxyuridine (BrdU) assay, and cell cycle progression was assessed through flow cytometry. The expression of cyclin A, cyclin‐dependent kinase 1 (cdk1), and cdk2 was examined using Western blotting. The expression of myoblast determination protein 1 (MyoD1) was examined through Western blotting and immunofluorescence staining. The LP‐PRP and LR‐PRP both promoted the proliferation of myoblasts and increased differential gene expression of myoblasts. Moreover, the LP‐PRP and LR‐PRP substantially upregulated the expression of cyclin A, cdk1, and cdk2. MyoD1 expression was induced in the LP‐PRP and LR‐PRP–treated myoblasts. Our results corroborate the finding that LP‐PRP and LR‐PRP have similar positive effects on myoblast proliferation and MyoD1 expression.
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.25666