The UFMylated ribosome-recognition protein SAYSD1 is predominantly expressed in spermatids but is dispensable for fertility in mice

SAYSVFN domain-containing protein 1 (SAYSD1) is an evolutionarily conserved membrane protein that has recently been identified as a ubiquitin-fold modifier 1 (UFM1)-conjugated ribosome-recognition protein that plays a critical role in translocation-associated quality control (TAQC). However, its exp...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2023-09, Vol.674, p.102-108
Main Authors: Chang, Lei, Fujii, Wataru, Yogo, Keiichiro
Format: Article
Language:English
Subjects:
Animals
Endoplasmic reticulum stress
Fertility
Male
Male fertility
Mammals
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mice
Mice, Knockout
Protein quality control
Ribosomal Proteins - genetics
Ribosomal Proteins - metabolism
Ribosomes
Semen
Spermatids - metabolism
Spermatogenesis
Spermatozoa - metabolism
Testis - metabolism
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Summary:SAYSVFN domain-containing protein 1 (SAYSD1) is an evolutionarily conserved membrane protein that has recently been identified as a ubiquitin-fold modifier 1 (UFM1)-conjugated ribosome-recognition protein that plays a critical role in translocation-associated quality control (TAQC). However, its expression and roles in mammals in vivo remain largely unknown. We found that SAYSD1 is predominantly expressed in round and elongating spermatids and localizes in the endoplasmic reticulum (ER) of mouse testes, but not in differentiated spermatozoa. Mice deficient in Saysd1 developed normally post-partum. Furthermore, Saysd1-deficient mice were fertile, with no apparent differences in sperm morphology or motility compared with wild-type sperm, although the cauda epididymis contained slightly less sperm. Expression of the ER stress markers spliced X-box binding protein 1s (XBP1s) and CCAAT/enhancer binding protein (C/EBP)-homologous protein (CHOP) in the testes was comparable between Saysd1-deficient and wild-type mice. These results suggested that SAYSD1 is involved in sperm production in mice but is dispensable for their development and fertility. •SAYSD1 is expressed in mouse spermatids and localized to the ER.•Saysd1-KO mice develop normally and are fertile.•The unfolding protein response is not increased in Saysd1-KO testes.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2023.06.085