A novel approach to diagnosing crystal-storing histiocytosis: utility of scanning electron microscopy for formalin-fixed paraffin-embedded tissue specimens

Crystal-storing histiocytosis (CSH) is a rare disorder that shows infiltration of histiocytes with an aberrant cytoplasmic accumulation of crystalline structures and is often accompanied by lymphoproliferative-plasma cell disorders (LP-PCD) as background diseases. The diagnosis of CSH requires ident...

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Bibliographic Details
Published in:Medical molecular morphology 2023-12, Vol.56 (4), p.297-302
Main Authors: Magara, Kazufumi, Takasawa, Akira, Kikuchi, Keisuke, Sugawara, Taro, Murakami, Taro, Kyuno, Daisuke, Ono, Yusuke, Takasawa, Kumi, Numata, Yasunao, Sasaki, Shigeru, Nakase, Hiroshi, Hasegawa, Tadashi, Osanai, Makoto
Format: Article
Language:English
Subjects:
Anatomy
Autopsy
Biopsy
Case Report
Electron microscopes
Formaldehyde
Histiocytosis
Light microscopy
Lymphocytes
Medicine
Medicine & Public Health
Molecular Medicine
Paraffin
Pathology
Scanning electron microscopy
Transmission electron microscopy
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Description
Summary:Crystal-storing histiocytosis (CSH) is a rare disorder that shows infiltration of histiocytes with an aberrant cytoplasmic accumulation of crystalline structures and is often accompanied by lymphoproliferative-plasma cell disorders (LP-PCD) as background diseases. The diagnosis of CSH requires identification of crystalline structures that accumulate in the infiltrating histiocytes, which may be challenging by optical microscopy alone. In this case report, we describe an atypical course of systemic CSH with multifocal fibrosclerosis of an unknown background disease that was diagnosed by ultrastructural observation, including transmission electron microscopy (TEM) and scanning electron microscopy (SEM), in pathological autopsy. In addition, crystalline structures were successfully identified by scanning electron microscopic observations using formalin-fixed and paraffin-embedded (FFPE) tissue from biopsy specimens taken before death. Since CSH was identified by SEM in a tiny biopsy specimen, observation of histiocytic infiltrative lesions by SEM using FFPE tissue may lead to early detection of and initiation of treatment for CSH.
ISSN:1860-1480
1860-1499
DOI:10.1007/s00795-023-00363-y