Loading…
Investigation of XPD, miR-145 and miR-770 expression in patients with end-stage renal disease
Background The effective maintenance of genome integrity and fidelity is vital for the normal function of our tissues and organs, and the prevention of diseases. DNA repair pathways maintain genome stability, and the adequacy of genes acting in these pathways is essential for disease suppression and...
Saved in:
Published in: | Molecular biology reports 2023-08, Vol.50 (8), p.6843-6850 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Background
The effective maintenance of genome integrity and fidelity is vital for the normal function of our tissues and organs, and the prevention of diseases. DNA repair pathways maintain genome stability, and the adequacy of genes acting in these pathways is essential for disease suppression and direct treatment responses. Chronic kidney disease is characterized by high levels of genomic damage. In this study, we examined the expression levels of the xeroderma pigmentosum group D (
XPD
) gene, which plays a role in the nucleotide excision repair (NER) repair mechanism, and the expression levels of
miR-145
and
miR-770
genes, which play a role in the regulation of the expression of the
XPD
gene, in hemodialysis patients with (n = 42) and without malignancy (n = 9) in pre- and post-dialysis conditions. We also evaluated these values with the clinical findings of the patients.
Methods & Results
Gene expression analysis was performed by real-time polymerase chain reaction (qRT-PCR). Compared to the individuals with normal kidney function (2.06 ± 0.32), the
XPD
gene expression was lower in the pre-dialysis condition both in hemodialysis patients without cancer (1.24 ± 0.18; p = 0.02) and in hemodialysis patients with cancer (0.82 ± 0.114; p = 0.001). On the other hand, we found that
miR-145
and
miR-770
expression levels were high in both groups. We also found that expression levels were affected by dialysis processes. A statistically significant positive correlation was found between
miR-145
and
mir770
expression levels in the pre-dialysis group of patients with (r=-0.988. p = 0.0001) and without (r=-0.934. p = 0.0001) malignancy.
Conclusions
Studies on DNA damage repair in the kidney will help develop strategies to protect kidney function against kidney diseases. |
---|---|
ISSN: | 0301-4851 1573-4978 |
DOI: | 10.1007/s11033-023-08608-w |