Phosphorylated bush sophora root polysaccharides protect the liver in duck viral hepatitis by preserving mitochondrial function

In order to ascertain the mechanism underlying the therapeutic efficacy of Bush sophora root polysaccharides (BSRPS) and phosphorylated Bush sophora root polysaccharides (pBSRPS) in the treatment of in duck viral hepatitis (DVH), an investigation was conducted to assess the protective impact of BSRP...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2023-08, Vol.245, p.125419-125419, Article 125419
Main Authors: Qiu, Tianxin, Shi, Yu, He, Miao, Wang, Wenjia, Meng, Jinwu, Ding, Jinxue, Wang, Weiran, Li, Siya, Li, Kun, Liu, Jiaguo
Format: Article
Language:English
Subjects:
DVH
Mitochondrial dysfunction
Phosphorylated bush sophora root polysaccharides
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to ascertain the mechanism underlying the therapeutic efficacy of Bush sophora root polysaccharides (BSRPS) and phosphorylated Bush sophora root polysaccharides (pBSRPS) in the treatment of in duck viral hepatitis (DVH), an investigation was conducted to assess the protective impact of BSRPS and pBSRPS against duck hepatitis A virus type 1 (DHAV-1) induced mitochondrial dysfunction both in vivo and vitro. The BSRPS underwent modification through the utilization of the sodium trimetaphosphate - sodium tripolyphosphate method, and was subsequently characterized though Fourier infrared spectroscopy and scanning electron microscopy. Following this, the degree of mitochondrial oxidative damage and dysfunction was described through the use of fluorescence probes and various antioxidative enzyme assay kits. Furthermore, the utilization of transmission electron microscopy facilitated the observation of alterations in the mitochondrial ultrastructure within the liver tissue. Our findings demonstrated that both BSRPS and pBSRPS effectively mitigated mitochondrial oxidative stress and conserved mitochondrial functionality, as evidenced by heightened antioxidant enzyme activity, augmented ATP production, and stabilized mitochondrial membrane potential. Meanwhile, the histological and biochemical examinations revealed that the administration of BSRPS and pBSRPS resulted in a reduction of focal necrosis and infiltration of inflammatory cells, thereby mitigating liver injury. Additionally, both BSRPS and pBSRPS exhibited the ability to maintain liver mitochondrial membrane integrity and enhance the survival rate of ducklings infected with DHAV-1. Notably, pBSRPS demonstrated superior performance in all aspects of mitochondrial function compared to BSRPS. The findings indicated that maintaining mitochondrial homeostasis is a crucial factor in DHAV-1 infections, and the administration of BSRPS and pBSRPS may mitigate mitochondrial dysfunction and safeguard liver function. •The phosphorylation modification of BSRPS was found to enhance its protective effects on mitochondria.•Mitochondrial dysfunction and oxidative stress induced by DHAV-1 were significantly reduced by pBSRPS.•The preservation of mitochondrial function by pBSRPS resulted in improved survival rates for DHAV-infected ducklings.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.125419