Multistrain Probiotics with Fructooligosaccharides Improve Middle Cerebral Artery Occlusion–Driven Neurological Deficits by Revamping Microbiota-Gut-Brain Axis

Recent burgeoning literature unveils the importance of gut microbiota in the neuropathology of post-stroke brain injury and recovery. Indeed, ingestion of prebiotics/probiotics imparts positive effects on post-stroke brain injury, neuroinflammation, gut dysbiosis, and intestinal integrity. However,...

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Bibliographic Details
Published in:Probiotics and antimicrobial proteins 2024-08, Vol.16 (4), p.1251-1269
Main Authors: Rahman, Ziaur, Bhale, Nagesh A., Dikundwar, Amol G., Dandekar, Manoj P.
Format: Article
Language:English
Subjects:
Applied Microbiology
Biomedical and Life Sciences
Brain injury
Caspase-3
Cerebral blood flow
Chemistry/Food Science
Digestive system
Digestive tract
Dysbacteriosis
Fructooligosaccharides
Gene expression
Glial fibrillary acidic protein
Intestinal microflora
Intestine
Ischemia
Lactobacillus reuteri
Life Sciences
Matrix metalloproteinase
Metalloproteinase
Microbiology
Microbiota
Neurological diseases
Neuronal-glial interactions
Nutrition
Prebiotics
Probiotics
Protein Science
rRNA 16S
Sensorimotor system
Stroke
Traumatic brain injury
Tumor necrosis factor-α
Zonula occludens-1 protein
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Summary:Recent burgeoning literature unveils the importance of gut microbiota in the neuropathology of post-stroke brain injury and recovery. Indeed, ingestion of prebiotics/probiotics imparts positive effects on post-stroke brain injury, neuroinflammation, gut dysbiosis, and intestinal integrity. However, information on the disease-specific preference of selective prebiotics/probiotics/synbiotics and their underlying mechanism is yet elusive. Herein, we examined the effect of a new synbiotic formulation containing multistrain probiotics ( Lactobacillus reuteri UBLRu-87, Lactobacillus plantarum UBLP-40, Lactobacillus rhamnosus UBLR-58, Lactobacillus salivarius UBLS-22, and Bifidobacterium breve UBBr-01), and prebiotic fructooligosaccharides using a middle cerebral artery occlusion (MCAO) model of cerebral ischemia in female and male rats. Three weeks pre-MCAO administration of synbiotic rescinded the MCAO-induced sensorimotor and motor deficits on day 3 post-stroke in rotarod, foot-fault, adhesive removal, and paw whisker test. We also observed a decrease in infarct volume and neuronal death in the ipsilateral hemisphere of synbiotic-treated MCAO rats. The synbiotic treatment also reversed the elevated levels/mRNA expression of the glial fibrillary acidic protein (GFAP), NeuN, IL-1β, TNF-α, IL-6, matrix metalloproteinase-9, and caspase-3 and decreased levels of occludin and zonula occludens-1 in MCAO rats. 16S rRNA gene-sequencing data of intestinal contents indicated an increase in genus/species of Prevotella ( Prevotella copri ), Lactobacillus ( Lactobacillus reuteri ), Roseburia , Allobaculum , and Faecalibacterium prausnitzii , and decreased abundance of Helicobacter , Desulfovibrio , and Akkermansia ( Akkermansia muciniphila ) in synbiotic-treated rats compared to the MCAO surgery group. These findings confer the potential benefits of our novel synbiotic preparation for MCAO-induced neurological dysfunctions by reshaping the gut-brain-axis mediators in rats.
ISSN:1867-1306
1867-1314
1867-1314
DOI:10.1007/s12602-023-10109-y