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Utilization of olive mill wastewater for selective production of lipids and carotenoids by Rhodotorula glutinis
Olive mill wastewater (OMW) is a zero-cost substrate for numerous value-added compounds. Although several studies on the production of lipids and carotenoids by Rhodotorula glutinis in OMW exist, none of them has specifically focused on the conditions for a target lipid or carotenoid. This study pre...
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| Published in: | Applied microbiology and biotechnology 2023-08, Vol.107 (15), p.4973-4985 |
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| container_title | Applied microbiology and biotechnology |
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| creator | Keskin, Abdulkadir Ünlü, Ayşe Ezgi Takaç, Serpil |
| description | Olive mill wastewater (OMW) is a zero-cost substrate for numerous value-added compounds. Although several studies on the production of lipids and carotenoids by
Rhodotorula glutinis
in OMW exist, none of them has specifically focused on the conditions for a target lipid or carotenoid. This study presents cultivation conditions that selectively stimulate the cell biomass, individual carotenoids and lipids. It was found that supplemental carbon and nitrogen sources as well as illumination affected cell biomass the most. High temperature, low initial pH, illumination, lack of urea and presence of glycerol stimulated the lipid synthesis. The highest total lipid content obtained in undiluted OMW supplemented with urea was 11.08 ± 0.17% (w/w) whilst it was 41.40 ± 0.21% (w/w) when supplemented with glycerol. Moreover, the main fatty acid produced by
R. glutinis
in all media was oleic acid, whose fraction reached 63.94 ± 0.58%. Total carotenoid yield was significantly increased with low initial pH, high temperature, illumination, certain amounts of urea, glycerol and cultivation time. Up to 192.09 ± 0.16 μg/g cell carotenoid yield was achieved. Torularhodin could be selectively produced at high pH, low temperature and with urea and glycerol supplementation. To selectively induce torulene synthesis, cultivation conditions should have low pH, high temperature and illumination. In addition, low pH, high temperature and urea supplementation served high production of β-carotene. Up to 85.40 ± 0.76, 80.67 ± 1.40 and 39.45 ± 0.69% of torulene, torularhodin and β-carotene, respectively, were obtained under selected conditions.
Key points
• Cultivation conditions selectively induced target carotenoids and lipids
• 41.40 ± 0.21% (w/w) lipid content and 192.09 ± 0.16 μg/g cell carotenoid yield were achieved
• Markedly high selectivity values for torularhodin and torulene were achieved |
| doi_str_mv | 10.1007/s00253-023-12625-x |
| format | article |
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Rhodotorula glutinis
in OMW exist, none of them has specifically focused on the conditions for a target lipid or carotenoid. This study presents cultivation conditions that selectively stimulate the cell biomass, individual carotenoids and lipids. It was found that supplemental carbon and nitrogen sources as well as illumination affected cell biomass the most. High temperature, low initial pH, illumination, lack of urea and presence of glycerol stimulated the lipid synthesis. The highest total lipid content obtained in undiluted OMW supplemented with urea was 11.08 ± 0.17% (w/w) whilst it was 41.40 ± 0.21% (w/w) when supplemented with glycerol. Moreover, the main fatty acid produced by
R. glutinis
in all media was oleic acid, whose fraction reached 63.94 ± 0.58%. Total carotenoid yield was significantly increased with low initial pH, high temperature, illumination, certain amounts of urea, glycerol and cultivation time. Up to 192.09 ± 0.16 μg/g cell carotenoid yield was achieved. Torularhodin could be selectively produced at high pH, low temperature and with urea and glycerol supplementation. To selectively induce torulene synthesis, cultivation conditions should have low pH, high temperature and illumination. In addition, low pH, high temperature and urea supplementation served high production of β-carotene. Up to 85.40 ± 0.76, 80.67 ± 1.40 and 39.45 ± 0.69% of torulene, torularhodin and β-carotene, respectively, were obtained under selected conditions.
Key points
• Cultivation conditions selectively induced target carotenoids and lipids
• 41.40 ± 0.21% (w/w) lipid content and 192.09 ± 0.16 μg/g cell carotenoid yield were achieved
• Markedly high selectivity values for torularhodin and torulene were achieved</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-023-12625-x</identifier><identifier>PMID: 37329489</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>beta Carotene ; Biomass ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Carbon sources ; Carotene ; Carotenoids ; Cottonseed oil mills ; Cultivation ; Environmental Biotechnology ; Fatty Acids ; Food industries wastewaters ; Glycerol ; High temperature ; Illumination ; Life Sciences ; Lipids ; Low temperature ; Methods ; Microbial Genetics and Genomics ; Microbiology ; Nitrogen sources ; Olea ; Oleic acid ; pH effects ; Physiological aspects ; Production processes ; Rhodotorula ; Rhodotorula glutinis ; Substrates ; Synthesis ; Urea ; Waste management ; Wastewater ; Yeast fungi ; β-Carotene</subject><ispartof>Applied microbiology and biotechnology, 2023-08, Vol.107 (15), p.4973-4985</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>COPYRIGHT 2023 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-ff020f80cb26585bf2cb58dffd5468400b0695b14438c99dce4f937cd2b173fe3</citedby><cites>FETCH-LOGICAL-c476t-ff020f80cb26585bf2cb58dffd5468400b0695b14438c99dce4f937cd2b173fe3</cites><orcidid>0000-0003-3722-9151 ; 0000-0001-6942-5777 ; 0000-0003-2272-7326</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2836652973/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2836652973?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>315,786,790,11715,27957,27958,36095,36096,44398,75252</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37329489$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Keskin, Abdulkadir</creatorcontrib><creatorcontrib>Ünlü, Ayşe Ezgi</creatorcontrib><creatorcontrib>Takaç, Serpil</creatorcontrib><title>Utilization of olive mill wastewater for selective production of lipids and carotenoids by Rhodotorula glutinis</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Olive mill wastewater (OMW) is a zero-cost substrate for numerous value-added compounds. Although several studies on the production of lipids and carotenoids by
Rhodotorula glutinis
in OMW exist, none of them has specifically focused on the conditions for a target lipid or carotenoid. This study presents cultivation conditions that selectively stimulate the cell biomass, individual carotenoids and lipids. It was found that supplemental carbon and nitrogen sources as well as illumination affected cell biomass the most. High temperature, low initial pH, illumination, lack of urea and presence of glycerol stimulated the lipid synthesis. The highest total lipid content obtained in undiluted OMW supplemented with urea was 11.08 ± 0.17% (w/w) whilst it was 41.40 ± 0.21% (w/w) when supplemented with glycerol. Moreover, the main fatty acid produced by
R. glutinis
in all media was oleic acid, whose fraction reached 63.94 ± 0.58%. Total carotenoid yield was significantly increased with low initial pH, high temperature, illumination, certain amounts of urea, glycerol and cultivation time. Up to 192.09 ± 0.16 μg/g cell carotenoid yield was achieved. Torularhodin could be selectively produced at high pH, low temperature and with urea and glycerol supplementation. To selectively induce torulene synthesis, cultivation conditions should have low pH, high temperature and illumination. In addition, low pH, high temperature and urea supplementation served high production of β-carotene. Up to 85.40 ± 0.76, 80.67 ± 1.40 and 39.45 ± 0.69% of torulene, torularhodin and β-carotene, respectively, were obtained under selected conditions.
Key points
• Cultivation conditions selectively induced target carotenoids and lipids
• 41.40 ± 0.21% (w/w) lipid content and 192.09 ± 0.16 μg/g cell carotenoid yield were achieved
• Markedly high selectivity values for torularhodin and torulene were achieved</description><subject>beta Carotene</subject><subject>Biomass</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Carbon sources</subject><subject>Carotene</subject><subject>Carotenoids</subject><subject>Cottonseed oil mills</subject><subject>Cultivation</subject><subject>Environmental Biotechnology</subject><subject>Fatty Acids</subject><subject>Food industries wastewaters</subject><subject>Glycerol</subject><subject>High temperature</subject><subject>Illumination</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Low temperature</subject><subject>Methods</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Nitrogen sources</subject><subject>Olea</subject><subject>Oleic acid</subject><subject>pH effects</subject><subject>Physiological aspects</subject><subject>Production processes</subject><subject>Rhodotorula</subject><subject>Rhodotorula glutinis</subject><subject>Substrates</subject><subject>Synthesis</subject><subject>Urea</subject><subject>Waste management</subject><subject>Wastewater</subject><subject>Yeast 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of olive mill wastewater for selective production of lipids and carotenoids by Rhodotorula glutinis</title><author>Keskin, Abdulkadir ; Ünlü, Ayşe Ezgi ; Takaç, Serpil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-ff020f80cb26585bf2cb58dffd5468400b0695b14438c99dce4f937cd2b173fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>beta Carotene</topic><topic>Biomass</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Carbon sources</topic><topic>Carotene</topic><topic>Carotenoids</topic><topic>Cottonseed oil mills</topic><topic>Cultivation</topic><topic>Environmental Biotechnology</topic><topic>Fatty Acids</topic><topic>Food industries wastewaters</topic><topic>Glycerol</topic><topic>High temperature</topic><topic>Illumination</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Low 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Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keskin, Abdulkadir</au><au>Ünlü, Ayşe Ezgi</au><au>Takaç, Serpil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utilization of olive mill wastewater for selective production of lipids and carotenoids by Rhodotorula glutinis</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2023-08-01</date><risdate>2023</risdate><volume>107</volume><issue>15</issue><spage>4973</spage><epage>4985</epage><pages>4973-4985</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Olive mill wastewater (OMW) is a zero-cost substrate for numerous value-added compounds. Although several studies on the production of lipids and carotenoids by
Rhodotorula glutinis
in OMW exist, none of them has specifically focused on the conditions for a target lipid or carotenoid. This study presents cultivation conditions that selectively stimulate the cell biomass, individual carotenoids and lipids. It was found that supplemental carbon and nitrogen sources as well as illumination affected cell biomass the most. High temperature, low initial pH, illumination, lack of urea and presence of glycerol stimulated the lipid synthesis. The highest total lipid content obtained in undiluted OMW supplemented with urea was 11.08 ± 0.17% (w/w) whilst it was 41.40 ± 0.21% (w/w) when supplemented with glycerol. Moreover, the main fatty acid produced by
R. glutinis
in all media was oleic acid, whose fraction reached 63.94 ± 0.58%. Total carotenoid yield was significantly increased with low initial pH, high temperature, illumination, certain amounts of urea, glycerol and cultivation time. Up to 192.09 ± 0.16 μg/g cell carotenoid yield was achieved. Torularhodin could be selectively produced at high pH, low temperature and with urea and glycerol supplementation. To selectively induce torulene synthesis, cultivation conditions should have low pH, high temperature and illumination. In addition, low pH, high temperature and urea supplementation served high production of β-carotene. Up to 85.40 ± 0.76, 80.67 ± 1.40 and 39.45 ± 0.69% of torulene, torularhodin and β-carotene, respectively, were obtained under selected conditions.
Key points
• Cultivation conditions selectively induced target carotenoids and lipids
• 41.40 ± 0.21% (w/w) lipid content and 192.09 ± 0.16 μg/g cell carotenoid yield were achieved
• Markedly high selectivity values for torularhodin and torulene were achieved</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>37329489</pmid><doi>10.1007/s00253-023-12625-x</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-3722-9151</orcidid><orcidid>https://orcid.org/0000-0001-6942-5777</orcidid><orcidid>https://orcid.org/0000-0003-2272-7326</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2023-08, Vol.107 (15), p.4973-4985 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2827263603 |
| source | ABI/INFORM Global; Springer Link |
| subjects | beta Carotene Biomass Biomedical and Life Sciences Biosynthesis Biotechnology Carbon sources Carotene Carotenoids Cottonseed oil mills Cultivation Environmental Biotechnology Fatty Acids Food industries wastewaters Glycerol High temperature Illumination Life Sciences Lipids Low temperature Methods Microbial Genetics and Genomics Microbiology Nitrogen sources Olea Oleic acid pH effects Physiological aspects Production processes Rhodotorula Rhodotorula glutinis Substrates Synthesis Urea Waste management Wastewater Yeast fungi β-Carotene |
| title | Utilization of olive mill wastewater for selective production of lipids and carotenoids by Rhodotorula glutinis |
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