Influence of trem-1 gene polymorphisms on cytokine levels during malaria by Plasmodium vivax in a frontier area of the Brazilian Amazon

•Infected individuals with rs6910730A allele had higher IL-6, IL-10, and IFN-γ levels.•IL-5, IL-6, IL-10, TNF-α, and IFN-γ were increased in rs2234237T and infected.•The rs2234246CT genotype group and infected had higher levels of TNF-α, and IFN-γ.•IFN-γ was higher in the infected group with rs47116...

Full description

Saved in:
Bibliographic Details
Published in:Cytokine (Philadelphia, Pa.) Pa.), 2023-09, Vol.169, p.156264-156264, Article 156264
Main Authors: de Jesus, Myrela C.S., Cerilo-Filho, Marcelo, Ramirez, Aina D.R., Menezes, Rubens A.O., Gomes, Margarete S.M., Cassiano, Gustavo C., Gurgel, Ricardo Q., Silva, José R.S., Moura, Tatiana R., Pratt-Riccio, Lilian R., Baptista, Andrea R.S., Storti-Melo, Luciane M., Machado, Ricardo L.D.
Format: Article
Language:English
Subjects:
Malaria
Plasmodium vivax
SNP
TREM-1
trem-1 gene
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Infected individuals with rs6910730A allele had higher IL-6, IL-10, and IFN-γ levels.•IL-5, IL-6, IL-10, TNF-α, and IFN-γ were increased in rs2234237T and infected.•The rs2234246CT genotype group and infected had higher levels of TNF-α, and IFN-γ.•IFN-γ was higher in the infected group with rs4711668C allele.•The trem-1 polymorphisms had no impact of the sTREM-1 levels. The immunopathology during malaria depends on the level of inflammatory response generated. In this scenario, the TREM-1 has been associated with the severity of infectious diseases and could play an important role in the inflammatory course of malaria. We aimed to describe the allelic and genotypic frequency of four polymorphisms in the trem-1 gene in Plasmodium vivax-infected patients and to verify the association of these polymorphisms with clinical and immunological factors in a frontier area of the Brazilian Amazon. We included 76 individuals infected with P. vivax and 144 healthy controls living in the municipality of Oiapoque, Amapá, Brazil. The levels of TNF-α, IL-10, IL-2, IL-4, IL-5, and IFN-γ were measured by flow cytometry, while IL-6, sTREM-1, and antibodies against PvMSP-119 were evaluated by ELISA. The SNPs were genotyped by qPCR technique. Polymorphisms analysis, allelic and genotype, frequencies, and HWE calculation were determined by x2 test in R Software. The association between the parasitemia, gametocytes, antibodies, cytokines, and sTREM-1 with the genotypes of malaria and control groups was performed using the Kruskal-Wallis test, these analyzes were conducted in SPSS Software, at 5% significance level. All SNPs were successfully genotyped. Allelic and genotypic distribution was in Hardy-Weinberg Equilibrium. Furthermore, several associations were identified between malaria and control groups, with increased levels of IL-5, IL-6, IL-10, TNF-α, and IFN-γ in the infected individuals with rs6910730A, rs2234237T, rs2234246T, rs4711668C alleles compared to the homozygous wild-type and heterozygous genotypes of the controls (p-value 
ISSN:1043-4666
1096-0023
DOI:10.1016/j.cyto.2023.156264