Identification of a unique intervillous cellular signature in chronic histiocytic intervillositis

Chronic histiocytic intervillositis (CHI) is a rare histopathological lesion in the placenta characterized by an infiltrate of CD68+ cells in the intervillous space. CHI is associated with adverse pregnancy outcomes such as miscarriage, fetal growth restriction, and (late) intrauterine fetal death....

Full description

Saved in:

Bibliographic Details
Published in:	Placenta (Eastbourne) 2023-08, Vol.139, p.34-42
Main Authors:	Krop, Juliette, van der Meeren, Lotte E., van der Hoorn, Marie-Louise P., Ijsselsteijn, Marieke E., Dijkstra, Kyra L., Kapsenberg, H., van der Keur, C., Cornish, Emily F., Nikkels, Peter G.J., Koning, Frits, Claas, Frans H.J., Heidt, Sebastiaan, Eikmans, Michael, Bos, Manon
Format:	Article
Language:	eng
Subjects:	Abortion, Spontaneous - metabolism Chorionic Villi - metabolism Female Histiocytes - pathology Humans Placenta - metabolism Placenta Diseases - pathology Pregnancy Pregnancy Outcome
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Chronic histiocytic intervillositis (CHI) is a rare histopathological lesion in the placenta characterized by an infiltrate of CD68+ cells in the intervillous space. CHI is associated with adverse pregnancy outcomes such as miscarriage, fetal growth restriction, and (late) intrauterine fetal death. The adverse pregnancy outcomes and a variable recurrence rate of 25–100% underline its clinical relevance. The pathophysiologic mechanism of CHI is unclear, but it appears to be immunologically driven. The aim of this study was to obtain a better understanding of the phenotype of the cellular infiltrate in CHI. We used imaging mass cytometry to achieve in-depth visualization of the intervillous maternal immune cells and investigated their spatial orientation in situ in relation to the fetal syncytiotrophoblast. We found three phenotypically distinct CD68+HLA-DR+CD38+ cell clusters that were unique for CHI. Additionally, syncytiotrophoblast cells in the vicinity of these CD68+HLA-DR+CD38+ cells showed decreased expression of the immunosuppressive enzyme CD39. The current results provide novel insight into the phenotype of CD68+ cells in CHI. The identification of unique CD68+ cell clusters will allow more detailed analysis of their function and could result in novel therapeutic targets for CHI. •CHI displays three phenotypically different intervillous macrophages.•The CHI macrophage cell clusters commonly express CD68, HLA-DR, and CD38.•CD68+HLA-DR + CD38+ macrophages are absent in the intervillous space of controls.•CD68+HLA-DR + CD38+ macrophages are in contact with SCT with reduced CD39 expression.
ISSN:	0143-4004 1532-3102