Stable isotope labeling differential glycans discovery in the serum of acute myocardial infarction by ultrahigh-performance liquid chromatography-quadrupole-Orbitrap high resolution mass spectrometry

Acute myocardial infarction (AMI) poses a grave threat to human life. However, most clinical biomarkers have limitations of low sensitivity and specificity. Therefore, screening novel glycan biomarkers with high sensitivity and specificity is crucial for the prevention and treatment of AMI. The nove...

Full description

Saved in:
Bibliographic Details
Published in:Analytica chimica acta 2023-07, Vol.1264, p.341269-341269, Article 341269
Main Authors: Li, Xi-Ling, Li, Yuxuan, Xiao, Shuyun, Li, Qingsong, Han, Chengqiang, Liu, Danyang, Cui, Tengfei, Rao, Xiyang, Todoroki, Kenichiro, Yang, Guang, Min, Jun Zhe
Format: Article
Language:English
Subjects:
Acute myocardial infarction
Biomarkers
Chromatography, High Pressure Liquid - methods
Chromatography, Liquid
Glycan biomarkers
Humans
Isotope Labeling - methods
Mass Spectrometry - methods
Polysaccharides - analysis
Pronase E
Stable isotope labeling
UHPLC-HRMS
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Acute myocardial infarction (AMI) poses a grave threat to human life. However, most clinical biomarkers have limitations of low sensitivity and specificity. Therefore, screening novel glycan biomarkers with high sensitivity and specificity is crucial for the prevention and treatment of AMI. The novel method of ultrahigh-performance liquid chromatography coupled to quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) with d0/d5-BOTC probe labeling for relative quantification of glycans based on Pronase E digestion was established to screen novel glycan biomarkers in the serum of 34 AMI patients relative to healthy volunteers. The monosaccharide model D-glucosamine was used to investigate the effectiveness of the derivatization; the limit of detection (S/N = 3) was 10 amol. The accuracy was verified based on the consistency of different theoretical molar ratios (d0/d5 = 1:2, 2:1) and intensity ratios following digestion of glycoprotein ribonuclease B. Expressions of H4N4F3SA, H4N6F2, H4N6SA, H4N6F3 and H5N4FSA in the serum were significantly different (p 
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2023.341269