Bifurcaria bifurcata extract exerts antioxidant effects on human Caco-2 cells

The present research study investigated the potential protective effect of Bifurcaria bifurcata extract on cell viability and antioxidant defences of cultured human Caco-2 cells submitted to oxidative stress induced by tert-butylhydroperoxide (tert-BOOH). Aqueous extracts were firstly characterized...

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Published in:Environmental research 2023-08, Vol.231 (Pt 1), p.116141-116141, Article 116141
Main Authors: Martínez, María-Aránzazu, Aedo, Hugo, Lopez-Torres, Bernardo, Maximiliano, Jorge-Enrique, Martínez-Larrañaga, María-Rosa, Anadón, Arturo, Martínez, Marta, Peteiro, Cesar, Cueto, Mercedes, Rubiño, Susana, Hortos, María, Ares, Irma
Format: Article
Language:English
Subjects:
Antioxidant defences
Antioxidants - metabolism
Antioxidants - pharmacology
B. bifurcata extract
Caco-2 Cells
Caspase 3 - metabolism
Humans
Oxidative Stress
Oxidative stress biomarkers
Phenolic and carbohydrate derivatives
Reactive Oxygen Species - metabolism
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Summary:The present research study investigated the potential protective effect of Bifurcaria bifurcata extract on cell viability and antioxidant defences of cultured human Caco-2 cells submitted to oxidative stress induced by tert-butylhydroperoxide (tert-BOOH). Aqueous extracts were firstly characterized in terms of total phenolic contents. Concentrations of reduced glutathione (GSH) and malondialdehyde (MDA), generation of reactive oxygen species (ROS), nitric oxide (NO) production, antioxidant enzymes activities [NADPH quinone dehydrogenase 1 (NQO1) and glutathione S-transferase (GST)], caspase 3/7 activity and gene expression linked to apoptosis, proinflammation and oxidative stress signaling pathways were used as markers of cellular oxidative status. B. bifurcata extract prevented the cytotoxicity, the decrease of GSH, the increase of MDA levels and the ROS generation induced by tert-BOOH. B. bifurcata extract prevented the significant decrease of NQO1 and GST activities, and the significant increase of caspase 3/7 activity induced by tert-BOOH. B. bifurcata extract also caused an over-expression of GSTM2, Nrf2 and AKT1 transcriptors, as well as reduced ERK1, JNK1, Bax, BNIP3, NFκB1, IL-6 and HO-1 gene expressions induced by tert-BOOH suggesting an increase in cellular resistance against oxidative stress. The results of the biomarkers analyzed show that treatment of Caco-2 cells with B. bifurcata extract enhance antioxidant defences, which imply an improved cell response to an oxidative challenge. B. bifurcata extract possesses strong antioxidant properties and may be a potential effective alternative to oxidant agents in the functional food industry. •Antioxidant activity of Bifurcaria bifurcata extract in Caco-2 cells.•B. bifurcata extract prevents NQO1 and GST decreased activities and GSH depletion induced by tert-BOOH.•B. bifurcata extract prevents MDA, ROS and NO increased levels induced by tert-BOOH.•B. bifurcata extract prevents caspase 3/7 activity induced by tert-BOOH.•B. bifurcata extract partly restraining apoptotic, inflammatory and oxidative stress pathways activated by tert-BOOH.
ISSN:0013-9351
1096-0953
DOI:10.1016/j.envres.2023.116141