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A novel carboxyl polymer-modified upconversion luminescent nanoprobe for detection of prostate-specific antigen in the clinical gray zonebase by flow immunoassay strip
•The whole assay process can be completed within 15 min.•We used a one-step reaction to complete the conversion of oil-phase UCNPs to aqueous-phase UCNP-COOH within 2 h by the carboxyl polymer embedding method.•Our t-PSA UCNPs strip has excellent accuracy, anti-interference and stability in the clin...
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Published in: | Methods (San Diego, Calif.) Calif.), 2023-07, Vol.215, p.10-16 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | •The whole assay process can be completed within 15 min.•We used a one-step reaction to complete the conversion of oil-phase UCNPs to aqueous-phase UCNP-COOH within 2 h by the carboxyl polymer embedding method.•Our t-PSA UCNPs strip has excellent accuracy, anti-interference and stability in the clinical gray zone (4–10 ng/mL) of PSA clinical application.•It is a portable analytical tool suitable for on-site analysis and rapid screening.•We validated this UCNP PSA strip in a large-scale clinical sample of 1397 patients, showing good consistency with the Roche chemiluminescence kit.
Prostate specific antigen (PSA) is a widely-used biomarker for the diagnosis, screening, and prognosis of prostate cancer (PCa). It is critical to develop a rapid and convenient method to accurately detect PSA levels, especially when the PSA levels are in the clinical gray area of 4–10 ng/mL. We developed a novel upconversion nanoparticle (UCNP)-based fluorescence lateral flow test strip for qualitatively and quantitatively detecting PSA. The carboxyl group-modified UCNPs (UCNP-COOH) were labeled with anti-PSA antibodies via 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as labeling probes to recognize PSA. The fluorescence intensity of the UCNP-probe was then measured with a laser fluorescence scanner. A total of 1397 serum and 20 fingertip blood samples were collected to validate the UCNP strip. A reliable correlation between the area ratio (TC), reflecting the fluorescence intensity of the test/control line, and the PSA concentration was observed (r = 0.9986). The dose-dependent luminescence enhancement showed good linearity in the PSA concentration range from 0.1 to 100.0 ng/mL with a detection limit of 0.1 ng/mL. Our UCNP POCT strip demonstrated excellent accuracy, anti-interference and stability in the gray zone (4–10 ng/mL) of PSA clinical application and outperformed other PSA test strips. The UCNP strip showed good consistency with the Roche chemiluminescence assay in 1397 serum samples. It also showed good performance for PSA detection using fingertip blood samples. This novel UCNP-based test strip could be a sensitive and reliable POCT assay to detect PSA, facilitating the diagnosis and surveillance of PCa. |
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ISSN: | 1046-2023 1095-9130 |
DOI: | 10.1016/j.ymeth.2023.05.001 |