Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 preventing acute liver injury in mice

BACKGROUND Bifidobacterium pseudolongum is widely exists in mammal gut and its abundance is associated with human and animal health. The present study aimed to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)‐induced acute liver injury (...

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Published in:Journal of the science of food and agriculture 2023-09, Vol.103 (12), p.5958-5969
Main Authors: Guo, Weiling, Cui, Shumao, Tang, Xin, Yan, Yongqiu, Xiong, Feifei, Zhang, Qiuxiang, Zhao, Jianxin, Mao, Bingyong, Zhang, Hao
Format: Article
Language:English
Subjects:
acute liver injury
Alanine
Alanine transaminase
Animal health
Animals
Antioxidants - metabolism
Bifidobacterium - metabolism
Bifidobacterium pseudolongum
Catalase
Catalase - metabolism
Chemical and Drug Induced Liver Injury - metabolism
Cytokines
Glutathione
Glutathione peroxidase
Humans
Hydrogen peroxide
Inflammation
Inflammation - metabolism
Inflammatory response
Injury analysis
Injury prevention
Interleukin 6
Interleukins
intestinal microbiota
Intestinal microflora
Intestine
Lipopolysaccharides
Liver
Liver - metabolism
liver metabolomics
Mammals - metabolism
Metabolism
Metabolites
Metabolomics
Metagenomics
Mice
Oxidation
Oxidative stress
Peroxidase
Phenylalanine
Probiotics
Riboflavin
Superoxide dismutase
Superoxide Dismutase - metabolism
Tricarboxylic acid cycle
Vitamin B
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Summary:BACKGROUND Bifidobacterium pseudolongum is widely exists in mammal gut and its abundance is associated with human and animal health. The present study aimed to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)‐induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles. RESULTS Bifidobacterium pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase and aspartate amino transferase activities. B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses (tumor necrosis factor‐α, interleukin‐1β, and interleukin‐6) and elevated antioxidative enzymes activities [total antioxidant capacity, superoxide dismutase, catalase, and glutathione peroxidase] in ALI mice by intervening in the Nf‐kβ and Nrf2 pathways, respectively. Bifidobacterium pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella and Ruminococcaceae UCG‐010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress. Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites‐related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (tricarboxylic acid cycle), and so on. Furthermore, riboflavin exposure could control the contents of malondialdehyde, superoxide dismutase, and catalase in hydrogen peroxide‐treated HepG2 cells. CONCLUSION Bifidobacterium pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS‐treated mice. Therefore, B. pseudolongum CCFM1253 could serves as a potential probiotic to ameliorate the host health. © 2023 Society of Chemical Industry.
ISSN:0022-5142
1097-0010
DOI:10.1002/jsfa.12665