Red biocolorant from endophytic Talaromyces minnesotensis: production, properties, and potential applications

Fungal colorants are gradually entering the global color market, given their advantages of being less harmful to human health, as well as having greater stability and biotechnological potential, compared to other natural sources. The present work concerns the isolation and identification of an endop...

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Published in:Applied microbiology and biotechnology 2023-06, Vol.107 (11), p.3699-3716
Main Authors: Sousa, Marina D. B., Pereira, Murilo L., Cruz, Felipe P. N., Romano, Luis H., Albuquerque, Yulli R., Correia, Ricardo O., Oliveira, Fernanda M., Primo, Fernando L., Baptista-Neto, Álvaro, Sousa, Cristina P., Anibal, Fernanda F., Moraes, Luiz Alberto B., Badino, Alberto C.
Format: Article
Language:English
Subjects:
Antimicrobial activity
Applied Microbial and Cell Physiology
Ascomycota
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Cold Temperature
Coloring agents
Coloring matter
Cytotoxicity
Enamel
Endophytes
Fluorescence
Food
Fungi
Fungiculture
Gelatin
Humans
Hydrogen-Ion Concentration
Life Sciences
Low temperature
Methods
Microbial Genetics and Genomics
Microbiology
Physiological aspects
Production processes
Properties
Saccharomyces cerevisiae
Stability analysis
Synthesis
Talaromyces
Temperature
Toxicity
Yeasts
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Summary:Fungal colorants are gradually entering the global color market, given their advantages of being less harmful to human health, as well as having greater stability and biotechnological potential, compared to other natural sources. The present work concerns the isolation and identification of an endophytic filamentous fungus, together with the chemical characterization and assessment of the fluorescence, toxicity, stability, and application potential of its synthesized red colorant. The endophytic fungus was isolated from Hymenaea courbaril , a tree from the Brazilian savannah, and was identified as Talaromyces minnesotensis by phenotypic and genotypic characterization. Submerged cultivation of the fungus resulted in the production of approximately 12 AU 500 of a red biocolorant which according to LC-DAD-MS analysis is characterized by being a complex mixture of molecules of the azaphilone class. Regarding cytotoxicity assays, activity against human hepatoblastoma (HepG2) cells was only observed at concentrations above 5.0 g L −1 , while antimicrobial effects against pathogenic bacteria and yeast occurred at concentrations above 50.0 g L −1 . The biocolorant showed high stability at neutral pH values and low temperatures (10 to 20 °C) and high half-life values (t 1/2 ), which indicates potential versatility for application in different matrices, as observed in tests using detergent, gelatin, enamel, paint, and fabrics. The results demonstrated that the biocolorant synthesized by Talaromyces minnesotensis has potential for future biotechnological applications. Key Points • An endophytic fungus, which was isolated and identified, synthesize a red colorant. • The colorant showed fluorescence property, low toxicity, and application potential. • The red biocolorant was highly stable at pH 8.0 and temperatures below 20°C. Graphical Abstract
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-023-12491-7