Characterization of the heteropolysaccharides produced by Liquorilactobacillus sicerae CUPV261 and Secundilactobacillus collinoides CUPV237 isolated from cider

Some lactic acid bacteria (LAB) strains isolated from alcoholic beverages are able to produce exopolysaccharides (EPS). The present work focuses on the physico-chemical characterization of the heteropolysaccharides (HePS) produced by Liquorilactobacillus sicerae CUPV261T (formerly known as Lactobaci...

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Bibliographic Details
Published in:International journal of food microbiology 2023-07, Vol.397, p.110199-110199, Article 110199
Main Authors: Puertas, Ana Isabel, Llamas-Arriba, Mª. Goretti, Etxebeste, Oier, Berregi, Iñaki, Pardo, Miguel Ángel, Prieto, Alicia, López, Paloma, Dueñas, Mª. Teresa
Format: Article
Language:English
Subjects:
Alcoholic Beverages - microbiology
Animals
Cider
Exopolysaccharides
Galactose
Gene clusters
Glucose
Heteropolysaccharides
Lactic acid bacteria
Liquorilactobacillus sicerae
Polysaccharides, Bacterial
Secundilactobacillus collinoides
Zebrafish
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Summary:Some lactic acid bacteria (LAB) strains isolated from alcoholic beverages are able to produce exopolysaccharides (EPS). The present work focuses on the physico-chemical characterization of the heteropolysaccharides (HePS) produced by Liquorilactobacillus sicerae CUPV261T (formerly known as Lactobacillus sicerae) and Secundilactobacillus collinoides CUPV237 (formerly known as Lactobacillus collinoides) strains isolated from cider. Genome sequencing and assembly enabled the identification of at least four putative HePS gene clusters in each strain, which correlated with the ability of both strains to secrete EPS. The crude EPS preparation from CUPV261T contained glucose, galactose and rhamnose, and that of CUPV237 was composed of glucose, galactose and N-acetylglucosamine. Both EPS were mixtures of HePS of different composition, with two major soluble components of average molecular weights (Mw) in the range of 106 and 104 g.mol−1. These HePS were resistant to gastric stress conditions in an in vitro model, and they significantly reduced zebrafish larvae mortality in an in vivo model of inflammatory bowel disease. •Strains CUPV261T and CUPV237 produce HePS of different composition and structure.•Both strains include in their genomes multiple potential EPS gene clusters.•EPS from both strains resisted simulated gastric stress conditions; they would reach small and large intestine.•Purified HePS preparations of both strains significantly reduced zebrafish mortality.
ISSN:0168-1605
1879-3460
DOI:10.1016/j.ijfoodmicro.2023.110199