MiR-29c-5p regulates the function of buffalo granulosa cells to induce follicular atresia by targeting INHBA

MicroRNAs (miRNAs) are involved in many physiological processes such as signal transduction, cell proliferation and apoptosis. Many studies have shown that miRNAs can regulate the process of follicular development. Our previous studies found that the expression of miR-29c-5p in buffalo atretic folli...

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Bibliographic Details
Published in:Theriogenology 2023-07, Vol.205, p.50-62
Main Authors: Cheng, Jiarui, Wei, Yaochang, Zhao, Ziwen, Xing, Qinghua, Gao, Ziyan, Cheng, Juanru, Yu, Chengqi, Pan, Yu, Yang, Yanyan, Shi, Deshun, Deng, Yanfei
Format: Article
Language:English
Subjects:
Activin A
Animals
Apoptosis - genetics
Buffalo granulosa cells
Buffaloes - genetics
Cell Proliferation
Female
Follicular atresia
Follicular Atresia - genetics
Granulosa Cells - metabolism
INHBA
Inhibin A
MicroRNAs - metabolism
MiR-29c-5p
Ovarian Follicle
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Summary:MicroRNAs (miRNAs) are involved in many physiological processes such as signal transduction, cell proliferation and apoptosis. Many studies have shown that miRNAs can regulate the process of follicular development. Our previous studies found that the expression of miR-29c-5p in buffalo atretic follicles was much higher than that in healthy follicles, suggesting that this miRNA may participate in the process of buffalo follicular atresia. In this study, we aim to explore to the role and molecular mechanisms of miR-29c-5p on the functions of buffalo granulosa cells (GCs). GCs cultured in vitro were transfected with miR-29c-5p mimics and its inhibitor, respectively, and it was found that the mimics significantly increased the apoptotic rate of GCs. They also inhibited the proliferation of GCs and the secretion of steroid hormones. The effect of the inhibitor was opposite to that of the mimics. MiR-29c-5p was subsequently shown to target the inhibin subunit beta A, (INHBA). Overexpression of INHBA could promote the production of activin A and inhibin A, and then reverse the effect of miR-29c-5p on buffalo GCs. In conclusion, these results suggest that miR-29c-5p promotes apoptosis and inhibits proliferation and steroidogenesis by targeting INHBA in buffalo GCs. This may ultimately promote atresia in buffalo follicles. •MiR-29c-5p expression is higher in atretic follicles than in healthy follicles.•MiR-29c-5p promotes apoptosis and inhibits proliferation on GCs of buffalo.•MiR-29c-5p inhibits the synthesis of PROG and E2 on GCs of buffalo.•MiR-29c-5p induces buffalo follicular atresia by targeting the INHBA 3′UTR region.•INHBA regulates the function of GCs by controlling the production of ACV-A and INH-A.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2023.04.013