Optimizing elicitation strategy of salicylic acid for flavonoid and phenolic production of fed-batch cultured Oplopanax elatus adventitious roots

Oplopanax elatus is a valuable medicinal plant, but its plant resource is lacking. Adventitious root (AR) culture of O. elatus is an effective way for the production of plant materials. Salicylic acid (SA) exerts enhancement effect on metabolite synthesis in some plant cell/organ culture systems. To...

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Bibliographic Details
Published in:Journal of biotechnology 2023-05, Vol.368, p.1-11
Main Authors: Yu, Shuo, Wu, Xiao-Han, Wang, Miao, Liu, Liang-Liang, Ye, Wei-Qi, Jin, Mei-Yu, Piao, Xuan-Chun, Lian, Mei-Lan
Format: Article
Language:English
Subjects:
Antioxidant activity
Antioxidants - metabolism
Economical elicitor
Elicitation strategy
Flavonoids
Improvement of metabolite synthesis
Oplopanax - chemistry
Oplopanax - metabolism
Phenols - chemistry
Salicylic Acid - pharmacology
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Summary:Oplopanax elatus is a valuable medicinal plant, but its plant resource is lacking. Adventitious root (AR) culture of O. elatus is an effective way for the production of plant materials. Salicylic acid (SA) exerts enhancement effect on metabolite synthesis in some plant cell/organ culture systems. To clarify the elicitation effect of SA on fed-batch cultured O. elatus ARs, this study investigated the effects of SA concentration, and elicitation time and duration. Results showed that flavonoid and phenolic contents, and antioxidant enzyme activity obviously increased when the fed-batch cultured ARs were treated with 100 μM SA for 4 days starting on day 35. Under this elicitation condition, total flavonoid and phenolic contents reached 387 rutin mg/g DW and 128 gallic acid mg/g DW, respectively, which were significantly (p 
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2023.04.004