Stereoselective behaviors and enantiomeric effects of paclobutrazol on microorganisms during Chinese cabbage pickling process

Studies on the differences between chiral pesticide enantiomers have caused widespread concern in the last decade. In the current work, the selective behaviors and different biological activities of paclobutrazol enantiomers during Chinese cabbage pickling process were evaluated. Results of degradat...

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Published in:Chirality (New York, N.Y.) N.Y.), 2023-06, Vol.35 (6), p.376-386
Main Authors: Chen, Min, Guo, Hao‐Ming, Di, Shan‐Shan, Zhao, Yue, Zhou, Dong‐Dong, Cao, Yi‐Wen, Tian, Pei, Yang, Zhong‐Hua, Zhao, Hui‐Yu
Format: Article
Language:English
Subjects:
Biological activity
Brassica
Brassica oleracea
Chinese cabbage
Community composition
Conversion
Degradation
enantiomeric effect
Enantiomers
Food contamination
Food safety
Health risks
high‐throughput sequencing
Humans
microorganism
Microorganisms
paclobutrazol
Pesticide residues
Pesticides
Pickling
Raw materials
RNA, Ribosomal, 16S
rRNA 16S
Stereoisomerism
Stereoselectivity
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Summary:Studies on the differences between chiral pesticide enantiomers have caused widespread concern in the last decade. In the current work, the selective behaviors and different biological activities of paclobutrazol enantiomers during Chinese cabbage pickling process were evaluated. Results of degradation kinetics indicated that when paclobutrazol reside in raw material (Chinese cabbage) and was introduced into the pickling process, the degradation rates of the two paclobutrazol enantiomers were significantly different, the half‐lives of (2R, 3R)‐paclobutrazol (R‐paclobutrazol) and (2S, 3S)‐paclobutrazol (S‐paclobutrazol) were 18.24 and 6.19 d, respectively. Besides, the conversion between the two enantiomers could also be observed, and the conversion rate of R‐paclobutrazol to S‐paclobutrazol was slower than that of reverse process. In addition, from the analysis of 16S rRNA and ITS sequencing, we inferred that the degradation of paclobutrazol was probably due to the presence of Pseudomonas and Serratia. Moreover, there has a significant difference in biological activity between R‐paclobutrazol and S‐paclobutrazol and shown an obviously enantiomeric effects on microbial community composition of pickling system. Besides, the analysis of microbial community displayed R‐paclobutrazol might inhibit the growth of Erwinia (a sort of plant pathogens). Results from this study served to enhance our understanding of chiral pesticide residues on food safety and the potential risks to human health. In this study, there was a significant stereoselective degradation and enantiomer conversion behavior of paclobutrazol, and enantiomeric bioactivity differences were generated in the composition and metabolic function of microorganisms.
ISSN:0899-0042
1520-636X
DOI:10.1002/chir.23549