The chemical structure of the O-polysaccharide isolated from the lipopolysaccharide of Pectobacterium brasiliense IFB5527, a phytopathogenic bacterium of high economic importance

Pectobacterium brasiliense is a widespread plant pathogenic bacterium classified to the Pectobacteriaceae family, which causes significant economic losses because of the developed soft rot and blackleg symptoms on potatoes and a wide spectrum of crops, vegetables, and ornamentals. One of the key vir...

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Published in:Carbohydrate research 2023-05, Vol.527, p.108806-108806, Article 108806
Main Authors: Szulta, Sylwia, Kowalczyk, Agnieszka, Czerwicka-Pach, Małgorzata, Motyka-Pomagruk, Agata, Śledź, Wojciech, Kaczyńska, Natalia, Łojkowska, Ewa, Kaczyński, Zbigniew
Format: Article
Language:English
Subjects:
Lipopolysaccharide
Lipopolysaccharides
O-polysaccharide structure
Pectobacterium - chemistry
Pectobacterium brasiliense
Polysaccharides - chemistry
Soft rot
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Summary:Pectobacterium brasiliense is a widespread plant pathogenic bacterium classified to the Pectobacteriaceae family, which causes significant economic losses because of the developed soft rot and blackleg symptoms on potatoes and a wide spectrum of crops, vegetables, and ornamentals. One of the key virulence factors is a lipopolysaccharide due to its involvement in efficient colonisation of plant tissues and overcoming the host defence mechanisms. Thus, we structurally characterised the O-polysaccharide from the LPS of P. brasiliense strain IFB5527 (HAFL05) using chemical methods followed by GLC and GLC-MS as well as 1D and 2D NMR spectroscopy. The analyses revealed that the polysaccharide repeating unit consists of Fuc, Glc, GlcN and an unusual N-formylated 6-deoxy amino sugar, Qui3NFo, and has the structure shown below: [Display omitted] [Display omitted] •The O-polysaccharide was isolated from Pectobacterium brasiliense IFB5527 (HAFL05).•The chemical structure was determined using chemical methods and NMR spectroscopy.•The OPS contains an unusual monosaccharide - N-formyl derivative of 3-amino-3-deoxy-d-quinovose (3-amino-3,6-dideoxy-d-glucose, Qui3N).
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2023.108806