Genetic analysis of Vibrio alginolyticus challenged by Fructus schisandrae reveals the mechanism of virulence genes

•1200 μg/mL F. schisandrae inhibited the growth of V.alginolyticus ND-01.•V. alginolyticus under high concentrations of F. schisandrae stress had insufficient ability in the whole biofilm formation process.•DEGs was involved in the following functional categories: metabolic process, single-organism...

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Published in:Gene 2023-06, Vol.870, p.147421-147421, Article 147421
Main Authors: Yi, Xin, Xu, XiaoJin, Chen, YuNong, Xu, Genhuang, Zhu, ZhiQin, Li, Huiyao, Shen, HaoYang, Lin, Mao, Zhao, Wenyu, Zheng, Jiang, Jiang, XingLong
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Fishes - genetics
Fructus schisandrae
Molecular mechanism
Reproducibility of Results
Transcriptome analysis
Vibrio alginolyticus
Vibrio alginolyticus - genetics
Virulence - genetics
Virulence gene
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Summary:•1200 μg/mL F. schisandrae inhibited the growth of V.alginolyticus ND-01.•V. alginolyticus under high concentrations of F. schisandrae stress had insufficient ability in the whole biofilm formation process.•DEGs was involved in the following functional categories: metabolic process, single-organism process, catalytic activity and localization. Due to the abusive use of antibiotics, bacterial resistance has become a global problem and poses severe threats to aquaculture. The drug-resistant diseases caused by Vibrio alginolyticus have caused significant economic losses to cultured marine fish. Fructus schisandrae is used to treat inflammatory diseases in China and Japan. There have been no reports of bacterial molecular mechanisms associated with F. schisandrae stress. In this study, the inhibiting effect of F. schisandrae on the growth of V. alginolyticus was detected to understand response mechanisms at the molecular level. The antibacterial tests were analyzed via next-generation deep sequencing technology (RNA sequencing, RNA-seq). Wild V. alginolyticus (CK) was compared with V. alginolyticus, F. schisandrae incubated for 2 h, and V. alginolyticus, F. schisandrae incubated for 4 h. Our results revealed that there were 582 genes (236 upregulated and 346 downregulated) and 1068 genes (376 upregulated and 692 downregulated), respectively. Differentially expressed genes (DEGs) were involved in the following functional categories: metabolic process, single-organism process, catalytic activity, cellular process, binding, membrane, cell part, cell, and localization. FS_2 h was compared with FS_4 h, and 21 genes (14 upregulated and 7 downregulated) were obtained. The RNA-seq results were validated by detecting the expression levels of 13 genes using quantitative real-time polymerase chain reaction (qRT-PCR). The qRT-PCR results matched those of the sequencing, which reinforced the reliability of the RNA-seq. The results revealed the transcriptional response of V. alginolyticus to F. schisandrae, which will provide new ideas for studying V. alginolyticus' complex virulence molecular mechanism and the possibility of developing Schisandra to prevent and treat drug-resistant diseases.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2023.147421