OsRbohI Regulates Rice Growth and Development via Jasmonic Acid Signalling

Abstract Reactive oxygen species (ROS) are highly reactive molecules, generated by nicotinamide adenine dinucleotide phosphate oxidases encoded by respiratory burst oxidase homologs. The functions of the OsRbohs gene family in rice are diverse and poorly understood. OsRbohI was recently identified a...

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Published in:Plant and cell physiology 2023-06, Vol.64 (6), p.686-699
Main Authors: Qi, Jiaxuan, Yang, Shuaiqi, Salam, Abdul, Yang, Chunyan, Khan, Ali Raza, Wu, Junyu, Azhar, Wardah, Gan, Yinbo
Format: Article
Language:English
Subjects:
Cyclopentanes - metabolism
Cyclopentanes - pharmacology
Gene Expression Regulation, Plant
Growth and Development
Oryza - metabolism
Oxylipins - metabolism
Oxylipins - pharmacology
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - metabolism
Reactive Oxygen Species - metabolism
Signal Transduction
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Summary:Abstract Reactive oxygen species (ROS) are highly reactive molecules, generated by nicotinamide adenine dinucleotide phosphate oxidases encoded by respiratory burst oxidase homologs. The functions of the OsRbohs gene family in rice are diverse and poorly understood. OsRbohI was recently identified as a newly evolved gene in the rice OsRbohs gene family. However, the function of OsRbohI in regulating rice growth is not yet reported. In this study, our results indicate that knockout (KO) OsRbohI mutants showed significantly shorter shoot and primary roots, along with lower ROS content than the control lines, whereas the overexpression (OE) lines displayed contrasting results. Further experiments showed that the abnormal length of the shoot and root is mainly caused by altered cell size. These results indicate that OsRbohI regulates rice shoot and root growth through the ROS signal. More importantly, RNA-seq analysis and jasmonic acid (JA) treatment demonstrated that OsRbohI regulates rice growth via the JA synthesis and signaling pathways. Compared with the control, the results showed that the KO mutants were more sensitive to JA, whereas the OE lines were less sensitive to JA. Collectively, our results reveal a novel pathway in which OsRbohI regulates rice growth and development by affecting their ROS homeostasis through JA synthesis and signaling pathway.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcad031