Fentanyl inhibits cell invasion and migration by modulating NF-κB activation in glioma

[Display omitted] •Fentanyl inhibits the invasion and migration of U87 and U251 cell in vitro.•Fentanyl alleviates the invasive character of glioma cells through mediating P65 activation rather than Mu Opioid Receptors.•The inhibitory effect of fentanyl on glioma was verified by subcutaneous tumor i...

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Published in:	Brain research 2023-06, Vol.1809, p.148356-148356, Article 148356
Main Authors:	Liu, Duan-Zheng, Xu, Shi-Yuan
Format:	Article
Language:	eng
Subjects:	Brain Neoplasms - metabolism Cell Line, Tumor Cell Movement Cell Proliferation Fentanyl Fentanyl - pharmacology Glioma Glioma - metabolism Humans Invasion Migration Narcotic Antagonists - pharmacology Neoplasm Invasiveness - genetics NF-kappa B - metabolism NF-κB Signal Transduction
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Summary:	[Display omitted] •Fentanyl inhibits the invasion and migration of U87 and U251 cell in vitro.•Fentanyl alleviates the invasive character of glioma cells through mediating P65 activation rather than Mu Opioid Receptors.•The inhibitory effect of fentanyl on glioma was verified by subcutaneous tumor in nude mice. Fentanyl is widely used for anesthesia and analgesia in cancer patients. Recent studies have revealed its anti-growth effect in several categories of cancer. Gliomas are the most common primary tumors in the central nervous system with poor prognosis. To investigate the effects of fentanyl on gliomas, glioma cells were treated with different concentrations of fentanyl both in vitro and in vivo. Consequences of proliferation and invasive phenotypes, and related protein expression were evaluated in two human glioma cell lines (U251 and U87). Naloxone, Mu Opioid Receptor (MOR) antagonist, was introduced into culture media to assess the involvement of MOR in Fentanyl-mediated changes. When compared with control group, it could be found that Fentanyl inhibited function of glioma cells only at high concentrations. Western blot and immunofluorescence results revealed that Fentanyl exerted its action via modulating NF-κB (P65) activation which is likely independent of MOR. Moreover, overexpression of P65 by transfection P65-expressing vector restored the invasion and migration of glioma cells, which were inhibited by Fentanyl. In summary, this study showed that opioid pain medication Fentanyl was capable of decreasing invasiveness of glioma cells at a high concentration both in vitro and in vivo, likely via modulating P65 activation.
ISSN:	0006-8993 1872-6240