Mealtime alcohol consumption suppresses skeletal muscle mTORC1 signaling in female mice

To determine whether alcohol consumed within the meal influences the feeding induced increase in mTORC1 signaling. Alcohol provided in the liquid diet was consumed by alcohol naïve, fasted, C57BL/6Hsd female mice and gastrocnemius was collected 1hr after the refeeding. Subsequent experiments determi...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 2023-05, Vol.566-567, p.111914-111914, Article 111914
Main Authors: Bridges, Blake O., Tice, Abigail L., Laudato, Joseph A., Gordon, Bradley S., Steiner, Jennifer L.
Format: Article
Language:English
Subjects:
Alcohol
Alcohol Drinking
Anabolic response
Animals
Ethanol
Ethanol - metabolism
Ethanol - pharmacology
Female
Meals
Mechanistic Target of Rapamycin Complex 1 - metabolism
Mice
Mice, Inbred C57BL
Muscle, Skeletal - metabolism
Phosphorylation
Skeletal muscle
TOR Serine-Threonine Kinases - metabolism
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Summary:To determine whether alcohol consumed within the meal influences the feeding induced increase in mTORC1 signaling. Alcohol provided in the liquid diet was consumed by alcohol naïve, fasted, C57BL/6Hsd female mice and gastrocnemius was collected 1hr after the refeeding. Subsequent experiments determined the extent to which changes in mTORC1 signaling persisted across the day. Compared with control mice, protein synthesis, mTORC1 (Ser2448), 4EBP1 (Ser65), S6K1 (Thr389), rpS6 (Ser240/244), Akt (Thr308), and ULK1 (Ser757) were lower in EtOH. Similar suppressive patterns were observed in the hours following consumption of alcohol containing food throughout the dark cycle. Higher peak blood alcohol concentrations induced by intraperitoneal injection of alcohol extended the time and magnitude of mTORC1 pathway suppression. Alcohol administered as part of the meal results in lower skeletal muscle mTORC1 signaling while subsequent models show that alcohol may influence this pathway across the day. •Alcohol lowers skeletal muscle mTORC1 signaling induced by simultaneous meal intake.•Alcohol inverts diurnal pattern of mTORC1 signaling in skeletal muscle.•Binge alcohol via injection results in different response than meal intake.
ISSN:0303-7207
1872-8057
DOI:10.1016/j.mce.2023.111914