Chloroacetonitrile exposure induces endoplasmic reticulum stress and affects spindle assembly in mouse oocytes

Chloroacetonitrile (CAN) is a halogenated acetonitrile often produced while disinfecting drinking water. Previous studies have shown that maternal exposure to CAN interferes with fetal development; however, the adverse effects on maternal oocytes remain unknown. In this study, in vitro exposure of m...

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Bibliographic Details
Published in:Food and chemical toxicology 2023-06, Vol.176, p.113736-113736, Article 113736
Main Authors: Qiao, Feng-Xin, Sun, Ming-Xin, Xu, Zhi-Ran, Liu, Yue-Cen, Chen, Yan-Zhu, Wang, Hai-Long, Qi, Zhong-Quan, Xu, Chang-Long, Liu, Yu
Format: Article
Language:English
Subjects:
Acetonitriles
Animals
Cell Cycle
Chloroacetonitrile
Endoplasmic Reticulum Stress
ER stress
Female
Mice
Oocytes
Reactive oxygen species
Spindle
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Summary:Chloroacetonitrile (CAN) is a halogenated acetonitrile often produced while disinfecting drinking water. Previous studies have shown that maternal exposure to CAN interferes with fetal development; however, the adverse effects on maternal oocytes remain unknown. In this study, in vitro exposure of mouse oocytes to CAN reduced maturation significantly. Transcriptomics analysis showed that CAN altered the expression of multiple oocyte genes, especially those associated with the protein folding process. CAN exposure induced reactive oxygen species production, accompanied by endoplasmic reticulum (ER) stress and increased glucose regulated protein 78, C/EBP homologous protein and activating transcription factor 6 expression. Moreover, our results indicated that spindle morphology was impaired after CAN exposure. CAN disrupted polo-like kinase 1, pericentrin and p-Aurora A distribution, which may be an origin inducer that disrupts spindle assemble. Furthermore, exposure to CAN in vivo impaired follicular development. Taken together, our findings indicate that CAN exposure induces ER stress and affects spindle assembly in mouse oocytes. [Display omitted]
ISSN:0278-6915
1873-6351
DOI:10.1016/j.fct.2023.113736