Antimicrobial effectiveness of different irrigation activation techniques on teeth with artificial internal root resorption and contaminated with Enterococcus faecalis: a confocal laser scanning,icroscopy analysis

This study aimed to compare the antibacterial efficacy of standard needle irrigation (SNI), EDDY, passive ultrasonic irrigation (PUI), photon-induced photoacoustic streaming (PIPS), and shock wave enhanced emission photoacoustic streaming (SWEEPS) activation on the teeth with simulated internal root...

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Bibliographic Details
Published in:Lasers in medical science 2023-03, Vol.38 (1), p.89-89, Article 89
Main Authors: Akdere, S. Koşumcu, Aydin, Z. Uğur, Erdönmez, D.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antiinfectives and antibacterials
Antimicrobial activity
Bacteria
Biofilms
Confocal microscopy
Contamination
Dental caries
Dental Pulp Cavity
Dental roots
Dentistry
Enterococcus faecalis
Humans
Incisors
Irrigation
Laser applications
Lasers
Lasers, Solid-State
Lavage
Medicine
Medicine & Public Health
Nitric acid
Optical Devices
Optics
Original Article
Photonics
Quantum Optics
Root Canal Irrigants - pharmacology
Root Canal Irrigants - therapeutic use
Root Canal Preparation - methods
Root canals
Root Resorption
Roots
Scanning microscopy
Shock waves
Sodium hypochlorite
Sodium Hypochlorite - pharmacology
Statistical analysis
Streaming
Teeth
Therapeutic Irrigation - methods
Variance analysis
Vortices
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Summary:This study aimed to compare the antibacterial efficacy of standard needle irrigation (SNI), EDDY, passive ultrasonic irrigation (PUI), photon-induced photoacoustic streaming (PIPS), and shock wave enhanced emission photoacoustic streaming (SWEEPS) activation on the teeth with simulated internal root resorption (IRR) and contaminated with Enterococcus faecalis ( E. faecalis ) using confocal laser scanning microscopy (CLSM) analyses. A total of 79 human maxillary central incisors with a single canal were selected. The canals were accessed, and then, the roots were split in the bucco-lingual direction. Artificial IRR cavities (depth of 0.8 mm and a diameter of 1.6 mm) were prepared using round burs and 20% nitric acid in the middle region of the root halves. The root halves were reconstructed with cyanoacrylate glue, and the canals were contaminated with a culture of E. faecalis for 30 days. Root canal preparation was performed using the ProTaper Next rotary files up to X5 and 2.5% NaOCl irrigation. Teeth were randomly assigned to five groups according to the irrigation activation method ( n  = 15): SNI, EDDY, PUI, PIPS, and SWEEPS. The final irrigation procedures were performed using a total of 6 mL of 2.5% NaOCl for each tooth with an activation time of 3 × 30 s. The canals were stained with LIVE/DEAD BacLight dye and analyzed with CLSM to determine the percentages of dead bacteria in the biofilm. Two-way ANOVA and post hoc Tukey tests were used for statistical analysis ( P   0.05). In irrigation activation methods other than PIPS, there was no significant difference in the percentage of dead bacteria between the coronal, middle, and apical regions of the roots ( P  > 0.05). A higher percentage of dead bacteria was found in the middle region compared to the apical region in the PIPS ( P  
ISSN:1435-604X
0268-8921
1435-604X
DOI:10.1007/s10103-023-03748-8