Intraperitoneally administered native and lauroyl-modified hyaluronan films: Pharmacokinetic and metabolism studies

Hyaluronan is being investigated extensively as a biocompatible and biodegradable material for use in biomedical applications. While the derivatization of hyaluronan broadens its potential therapeutic use, the pharmacokinetics and metabolization of the derivatives must be thoroughly investigated. Th...

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Published in:Carbohydrate polymers 2023-01, Vol.299, p.120201-120201, Article 120201
Main Authors: Brtková, Barbora, Hermannová, Martina, Chmelař, Josef, Nešporová, Kristina, Kocurková, Anna, Kubala, Lukáš, Ambrožová, Gabriela, Velebný, Vladimír, Šimek, Matěj
Format: Article
Language:English
Subjects:
Acylation
Chromatography, Liquid
Degradation
Fatty Acids, Nonesterified
Hyaluronan derivatives
Hyaluronic Acid
Implants
In-vivo evaluation
Isotopes
Stable isotope
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Summary:Hyaluronan is being investigated extensively as a biocompatible and biodegradable material for use in biomedical applications. While the derivatization of hyaluronan broadens its potential therapeutic use, the pharmacokinetics and metabolization of the derivatives must be thoroughly investigated. The fate of intraperitoneally-applied native and lauroyl-modified hyaluronan films with varying degrees of substitution was investigated in-vivo employing an exclusive stable isotope-labelling approach and LC-MS analysis. The materials were gradually degraded in peritoneal fluid, lymphatically absorbed, preferentially metabolized in the liver and eliminated without any observable accumulation in the body. Hyaluronan acylation prolongs its presence in the peritoneal cavity depending on the degree of substitution. The safety of acylated hyaluronan derivatives was confirmed via a metabolic study that revealed its degradation into non-toxic metabolites, i.e. native hyaluronan and free fatty acid. Stable isotope-labelling with LC-MS tracking comprises a high-quality procedure for the investigation of the metabolism and biodegradability of hyaluronan-based medical products in-vivo. [Display omitted]
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2022.120201