An angled-shape tip-based strategy for highly sensitive proteomic profiling of a low number of cells

Profiling proteins plays an essential role in understanding the functions and dynamic networks in biological systems. Mass spectrometry-based proteomic analysis commonly requires multistep sample processing, which results in severe sample loss. Although the recently developed microproteomic strategi...

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Bibliographic Details
Published in:Analytical methods 2023-03, Vol.15 (9), p.1215-1222
Main Authors: Zhao, Hongxian, Chen, Yongle, Li, Hang, Zhang, Yangjun, Zhang, Wanjun, Qin, Weijie
Format: Article
Language:English
Subjects:
Biological research
Flow cytometry
HeLa Cells
Humans
Lysis
Mass spectrometry
Mass spectroscopy
Microfluidics
Peptides
Protein adsorption
Proteins
Proteins - analysis
Proteomics
Proteomics - methods
Reproducibility of Results
Sample preparation
Workflow
Citations: Items that this one cites
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Summary:Profiling proteins plays an essential role in understanding the functions and dynamic networks in biological systems. Mass spectrometry-based proteomic analysis commonly requires multistep sample processing, which results in severe sample loss. Although the recently developed microproteomic strategies have substantially reduced sample loss droplet microfluidic technology, specialized equipment and well-trained personnel are needed, which may limit their wide adoption. Here, we report an angled-shape tip-based strategy for rapid sample preparation and sensitive proteomic profiling of small cell populations (
ISSN:1759-9660
1759-9679
DOI:10.1039/d2ay01884e