The antibiotic resistome in Escherichia coli isolated from human, food, and animal sources

The aim of this study was to analyze and compare the prevalence and distribution of resistance genes in Escherichia coli genomes isolated from human clinical samples and animal-based foods worldwide. We download from NCBI Pathogen Detection Database the corresponding metadata of the 7,123 E. coli ge...

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Bibliographic Details
Published in:Journal of applied microbiology 2023-02, Vol.134 (2)
Main Authors: Rodrigues, Isadora de Alcântara, Ferrari, Rafaela Gomes, Panzenhagen, Pedro, Pereira Dos Santos, Anamaria Mota, Rodrigues, Grazielle Lima, Junior, Carlos Adam Conte, Mano, Sergio Borges
Format: Article
Language:English
Subjects:
Aminoglycosides
Animals
Anti-Bacterial Agents - pharmacology
beta-Lactamases - genetics
Escherichia coli - genetics
Escherichia coli Infections - epidemiology
Escherichia coli Proteins - genetics
Humans
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Summary:The aim of this study was to analyze and compare the prevalence and distribution of resistance genes in Escherichia coli genomes isolated from human clinical samples and animal-based foods worldwide. We download from NCBI Pathogen Detection Database the corresponding metadata of the 7,123 E. coli genome to access the information about the antimicrobial resistance gene content. The geographic location and the source of isolation were also obtained and compiled with the antimicrobial resistance gene for statistical analysis, results and discussion. Our criteria considered four groups for analyzing the antimicrobial resistance gene distribution. The first group of genomes from invasive clinical human (ICH) samples from countries with Human Development Index (HDI) ≥ 0.850; the second group of ICH from countries with an HDI ≤ 0.849; the third group of animal-based foods (ABF) from countries with HDI ≥ 0.850 and the fourth group of ABFs from countries with HDI ≤ 0.849. The most prevalent genes in the first group were blaCTX-M-134 (96.53%) and blaCTX-M-27 (86.35%). In the second group, ere(A) (95.96%), soxS (94.49%), qepA8 (90.81%), blaCTX-M-15 (85.66%), and fosA3 (80.88%). In the third group, the most frequently detected were aadA12 (98.5%), ant(3") (89.92%), and blaCARB-2 (87.2%). In the fourth group, aadA12 and aac(3)-IV were identified in 100% of the analyzed genomes. It was clear that the use of aminoglycosides in animal production is increasing the selective pressure on micro-organisms in both groups of countries since genes linked to aminoglycoside resistance are related to E. coli from ABF samples. The genomic profile of E. coli from HDI ≥ 0.850 countries indicates a selective pressure aimed at cephalosporins given the high prevalence in both sources.
ISSN:1365-2672
1365-2672
DOI:10.1093/jambio/lxac059