AjTGFβ alleviates V. splendidus-induced inflammation through SMADs pathway in Apostichopus japonicus

The inhibition of inflammatory response is an essential process to control the development of inflammation and is an important step to protect the organism from excessive inflammatory damage. As a pleiotropic cytokine, transforming growth factor beta (TGF-β) plays a regulatory role in inhibiting inf...

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Published in:Fish & shellfish immunology 2023-03, Vol.134, p.108593-108593, Article 108593
Main Authors: zhen, Zhang, wenwen, Ye, guanghui, Han, chenghua, Li, zhimeng, Lv
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Collagens
Inflammatory response
Invertebrates - classification
Invertebrates - genetics
Invertebrates - immunology
Models, Molecular
Phylogeny
Protein Structure, Tertiary
Sequence Alignment
Smad Proteins - metabolism
SMADs
Stichopus - classification
Stichopus - genetics
Stichopus - immunology
Stichopus - microbiology
Transforming growth factor beta (TGF-β)
Transforming Growth Factor beta - chemistry
Transforming Growth Factor beta - genetics
Transforming Growth Factor beta - immunology
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Summary:The inhibition of inflammatory response is an essential process to control the development of inflammation and is an important step to protect the organism from excessive inflammatory damage. As a pleiotropic cytokine, transforming growth factor beta (TGF-β) plays a regulatory role in inhibiting inflammation in vertebrates. To investigate the role of TGF-β in the regulation of inflammation in invertebrates, we cloned and characterized the TGF-β gene from Apostichopus japonicus via rapid amplification of cDNA ends, and the sample was designated as AjTGF-β. For Vibrio splendidus-challenged sea cucumbers, the expression of AjTGF-β mRNAs in coelomocytes decreased at 96 h (0.27-fold), which was contrary to the trend of inflammation. AjTGF-β was expressed in all tissues with the highest expression in the body wall. When AjTGF-β was knocked down by using small interfering RNA (siRNA-KD) to 0.45-fold, AjSMAD 2/3 and AjSMAD6 were downregulated to 0.32- and 0.05-fold compared with the control group, respectively. Furthermore, when the damaged sea cucumber was challenged by V. splendidus co-incubated with rAjTGF-β, the damage area had no extensive inflammation, and damaged repair appeared at 72 h compared with the Vs + BSA group, in which the expression of AjSMAD 2/3 was upregulated by 1.35-fold. Under this condition, AjSMAD 2/3 silencing alleviated rAjTGF-β-induced damage recovery. Moreover, rAjTGF-β slightly induced the collagen I expression from 6.13 ng/mL to 7.84 ng/mL, and collagen III was upregulated from 6.23 ng/mL to 6.89 ng/mL compared with the Vs + BSA group. This finding indicates that AjTGF-β negatively regulated the inflammatory progress and accelerated the repair of damage by AjSMADs to regulate the collagens expression. •The first TGF-β was identified from Apostichopus japonicus.•AjTGF-β mRNA levels displayed higher abundance in body wall.•rAjTGF-β accelerate the sea cucumber inflammation damaged repair.•AjSMAD 2/3 silencing alleviated rAjTGF-β-induced damage recovery.•rAjTGF-β was slightly induced the collagen I/III expression.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2023.108593