Therapeutic effects of L‐arginine, L‐carnitine, and mesenchymal stem cell‐conditioned medium on endometriosis‐induced oocyte poor quality in an experimental mouse model

Aim The present study aimed to explore the potential ameliorative effects of L‐arginine (LA), L‐carnitine (LC), and bone marrow mesenchymal stem cell‐conditioned medium (BMSC‐CM) on endometriosis (EMS) model in vivo and in vitro. Methods The animals were divided into two main groups, normal and EMS‐...

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Published in:The journal of obstetrics and gynaecology research 2023-04, Vol.49 (4), p.1180-1188
Main Authors: Kalehoei, Eshrat, Moradi, Mojtaba, Azadbakht, Mehri, Zhaleh, Hossein, Abadi, Samaneh Azizi Lal, Mahdiuni, Hamid, Gharzi, Ahmad
Format: Article
Language:English
Subjects:
Animals
Antioxidants - pharmacology
Arginine - pharmacology
Blastocyst
bone marrow mesenchymal stem cell‐conditioned medium
Carnitine
Carnitine - pharmacology
Culture Media, Conditioned - pharmacology
Embryogenesis
Embryonic Development
Endometriosis
Endometriosis - drug therapy
Female
female infertility
Fertilization in Vitro - methods
Humans
In vitro fertilization
In Vitro Oocyte Maturation Techniques - methods
Infertility
Mesenchymal Stem Cells
Mice
Nitric oxide
oocyte quality
Oocytes
Oxidative stress
Pregnancy
Reproductive technology
Stem cells
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Summary:Aim The present study aimed to explore the potential ameliorative effects of L‐arginine (LA), L‐carnitine (LC), and bone marrow mesenchymal stem cell‐conditioned medium (BMSC‐CM) on endometriosis (EMS) model in vivo and in vitro. Methods The animals were divided into two main groups, normal and EMS‐induced mice. Normal and EMS‐induced groups were injected with or without LA (250 mg/kg), LC (250 mg/kg), and BMSC‐CM (a final volume of 100 μL of CM/mouse). At the end of the study, the level of total antioxidant capacity (TAC), nitric oxide (NO), and total oxidative status (TOS) were measured in plasma. Furthermore, immature oocytes were collected from two groups and cultured in a maturation medium. Subsequently, the rates of in vitro maturation, in vitro fertilization (IVF), and in vitro embryonic development were evaluated. Results The results revealed that administration of LA, LC, and BMSC‐CM ameliorated the oxidative status through maintaining TAC and alleviating TOS and NO levels. More importantly, the maturation and fertilization rates, blastocyst development, and total blastocyst cell numbers significantly increased in LA, LC, and BMSC‐CM‐administrated groups compared to the control group. In both the normal and EMS groups, the highest IVF, cleavage, and blastocyst percentages were associated with BMSC‐CM treatment (p 
ISSN:1341-8076
1447-0756
DOI:10.1111/jog.15569