NMR metabolomics analysis of Escherichia coli cells treated with Turkish propolis water extract reveals nucleic acid metabolism as the major target

Propolis is a resinous bee product containing several hundred biologically active compounds. Although the antibacterial activity of propolis has been demonstrated in many in vitro studies, less is known about its mode of action. In this study, we aimed to shed some light on the antibacterial mechani...

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Bibliographic Details
Published in:Journal of applied microbiology 2023-01, Vol.134 (1)
Main Authors: Elgin, E Sonay, Çatav, Ş Serter, Babayeva, Anara, Kim, Hakbeom, Dibek, Esra, Çöl, Bekir, Chae, Young Kee, Kıvrak, İbrahim
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Escherichia coli
Magnetic Resonance Spectroscopy - methods
Metabolomics - methods
Nucleic Acids - metabolism
Propolis - chemistry
Propolis - pharmacology
Water - metabolism
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Summary:Propolis is a resinous bee product containing several hundred biologically active compounds. Although the antibacterial activity of propolis has been demonstrated in many in vitro studies, less is known about its mode of action. In this study, we aimed to shed some light on the antibacterial mechanism of action of propolis against Escherichia coli BW25113 using a nuclear magnetic resonance (NMR) based metabolomics approach. E. coli BW25113 cells were subjected to different sub-lethal concentrations (0, 2, 4, and 6 mg/mL) of Turkish propolis water extract (PWE). The 500-MHz 1H NMR spectroscopy was then employed to ascertain the metabolic profiles of E. coli extracts. A total of 52 metabolites were identified from the NMR spectra, belonging to 17 main classes, such as amino acids and peptides, purines, and fatty acids. Twelve out of these 52 metabolites displayed remarkable changes at all tested PWE concentrations when compared to control conditions (P 
ISSN:1365-2672
1365-2672
DOI:10.1093/jambio/lxac031