Purification and characterization of Taxol and 10‐Deacetyl baccatin III from the bark, needles, and endophytes of Taxus baccata by preparative high‐performance liquid chromatography, ultra‐high‐performance liquid chromatography‐mass spectrometry, and nuclear magnetic resonance

Taxol and 10‐Deacetyl baccatin III are major taxanes in the bark, needles, and endophytes of Taxus baccata. The current study aimed to develop a process for their separation from different matrices. Crude taxoid was prepared by extraction of samples with methanol, followed by partitioning with dichl...

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Bibliographic Details
Published in:Journal of separation science 2023-03, Vol.46 (6), p.e2200841-n/a
Main Authors: Nurullah, Md, Usmani, Zakiya, Ahmad, Sheeraz, Panda, Bibhu Prasad, Amin, Saima, Mir, Showkat Rasool
Format: Article
Language:English
Subjects:
10‐Deacetyl baccatin III
Biotransformation
Chromatography
Chromatography, High Pressure Liquid
Dichloromethane
endophytes
Endophytes - metabolism
Flow velocity
Hexanes
Liquid chromatography
Magnetic Resonance Spectroscopy
Mass Spectrometry
Methanol
Methanol - metabolism
Needles
NMR
Nuclear magnetic resonance
Paclitaxel
Paclitaxel - chemistry
Plant Bark - chemistry
Scientific imaging
Taxoids - analysis
Taxol
Taxus
Taxus baccata
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Summary:Taxol and 10‐Deacetyl baccatin III are major taxanes in the bark, needles, and endophytes of Taxus baccata. The current study aimed to develop a process for their separation from different matrices. Crude taxoid was prepared by extraction of samples with methanol, followed by partitioning with dichloromethane and precipitation with hexane. Analytical high‐performance liquid chromatography involved isocratic elution on C18 column (4.6 × 250 mm, 5 μm) with methanol‐water (70:30 v/v) at a flow rate of 1 ml/min. Injection volume was 20 μl and detection was carried out at 227 nm. The content of Taxol and 10‐Deacetyl baccatin III in bark, needles and endophytic culture broth was 11.19 and 1.75 μg/mg; 11.19 and 1.75 μg/mg; and 2.80 and 0.22 μg/L, respectively. Preparative high‐performance liquid chromatography was done on C18 column (10 × 250 mm, 5 μm) at a flow rate of 10 ml/min. About 20 g crude taxoid was processed in 
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202200841