Investigating the Effects of Biogenic Zinc Oxide Nanoparticles Produced Using Papaver somniferum Extract on Oxidative Stress, Cytotoxicity, and the Induction of Apoptosis in the THP-1 Cell Line

  This study investigated the effect of novel zinc oxide nanoparticles (ZnO NPs) biosynthesized employing Papaver somniferum leaf on oxidative stress, necrosis, and apoptosis in the leukemia cancer THP-1 cell. The obtained ZnO was examined using SEM, AFM, and TEM microscopy, which revealed an irregu...

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Bibliographic Details
Published in:Biological trace element research 2023-10, Vol.201 (10), p.4697-4709
Main Authors: Kadhim, Afraa Ali, Abbas, Nahida R., Kadhum, Hussam H., Albukhaty, Salim, Jabir, Majid S., Naji, Amel Muhson, Hamzah, Sawsan S., Mohammed, Mustafa K. A., Al-Karagoly, Hassan
Format: Article
Language:English
Subjects:
Annexin V
Anticancer properties
Antitumor agents
Apoptosis
Atomic force microscopy
Biochemistry
Biological stress
Biomedical and Life Sciences
Biotechnology
Cancer
Cell death
Cell proliferation
Cells
Cytotoxicity
Flow cytometry
L-Lactate dehydrogenase
Lactate
Lactate dehydrogenase
Leukemia
Life Sciences
Microscopy
Nanoparticles
Necrosis
Nutrition
Oncology
Oxidative stress
Papaver somniferum
Proliferation
Reactive oxygen species
Zinc
Zinc oxide
Zinc oxides
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Summary:  This study investigated the effect of novel zinc oxide nanoparticles (ZnO NPs) biosynthesized employing Papaver somniferum leaf on oxidative stress, necrosis, and apoptosis in the leukemia cancer THP-1 cell. The obtained ZnO was examined using SEM, AFM, and TEM microscopy, which revealed an irregular spherical morphology with a size ranging from 20 to 30 nm, and the UV–vis absorbance revealed a strong absorption peak in the range of 360–370, nm confirming the production of ZnO NPs. THP-1 cells were subjected to an MTT, an EdU proliferation, a lactate dehydrogenase release tests, a reactive oxygen species (ROS) induction experiment, a DAPI staining detection assay, and a flow cytometric analysis for Annexin V to measure the effects of ZnO NPs on cancer cell growth inhibition, apoptosis, and necrosis. Our results show that ZnO NPs inhibit THP-1 line in a concentration-dependent pattern. It was observed that ZnO NPs triggered necrosis (cell death) and apoptosis in the cell line. ZnO NPs massively improved the formation of intracellular ROS, which is crucial in deactivating the development of leukemic cells. In conclusion, ZnO nanoparticles synthesized using Papaver somniferum extract have the ability to inhibit proliferation leukemic cancer cells, making them potential anticancer agents.
ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-023-03574-7