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CDKL3 Promotes Non-small Cell Lung Cancer by Suppressing Autophagy Via Activation of PI3K/Akt/mTOR Pathway
This study aimed to assess the role of cyclin-dependent kinase-like 3 ( CDKL3 ) in the progression of non-small cell lung cancer (NSCLC) as well as the underlying mechanisms. Western blot and qRT-PCR were utilized to analyze CDKL3 expression in 30 pairs of NSCLC and paraneoplastic tissues. A549 cell...
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Published in: | Molecular biotechnology 2023-09, Vol.65 (9), p.1421-1431 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | This study aimed to assess the role of cyclin-dependent kinase-like 3 (
CDKL3
) in the progression of non-small cell lung cancer (NSCLC) as well as the underlying mechanisms. Western blot and qRT-PCR were utilized to analyze
CDKL3
expression in 30 pairs of NSCLC and paraneoplastic tissues. A549 cells with CDKL3 knockdown and PC9 cells with CDKL3 overexpression were constructed by infecting cells with lentiviruses expressing shRNA of
CDKL3
and expressing a full-length
CDKL3
mRNA, respectively. The CCK-8 assay, flow cytometry, wound healing assay, and Transwell assay were carried out to detect cell viability, apoptosis, migration, and invasion, respectively. Autophagosome morphology was observed by electron microscopy experiments, the expression of key components of the PI3K/Akt/mTOR pathway was examined via Western blot and their mRNA expression levels were determined. Besides, the stably infected NSCLC cells with reduced expression or overexpression of
CDKL3
were inoculated into the right-back flank of mice to generate tumors. The results showed that
CDKL3
expression was dramatically increased in NSCLC tissues. Moreover,
CDKL3
promoted the viability and migration of NSCLC cells by suppressing autophagy in vitro and in vivo
.
In addition,
CDKL3
might modulate PI3K/Akt/mTOR signaling in NSCLC. Overall,
CDKL3
might promote NSCLC cell viability and metastasis by inhibiting autophagy and activating the PI3K/Akt/mTOR signaling pathway. |
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ISSN: | 1073-6085 1559-0305 |
DOI: | 10.1007/s12033-023-00656-8 |