Purification, structural elucidation and biological activities of exopolysaccharide produced by the endophytic Penicillium javanicum from Millettia speciosa Champ

An acid polysaccharide, named HP, was produced by endophytic Penicillium javanicum MSC-R1 isolated from southern medicine Millettia speciosa Champ. The molecular weight of HP was 37.8 kDa and consisted of Ara f, Galр, Glcр, Manр, and GlcрA with a molar ratio of 1.09: 3.47: 68.48: 16.59: 8.85. The gl...

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Published in:Journal of biotechnology 2023-01, Vol.362, p.54-62
Main Authors: Lai, Lin-Hao, Zong, Min-Hua, Huang, Zhi, Ni, Zi-Fu, Xu, Pei, Lou, Wen-Yong
Format: Article
Language:English
Subjects:
Animals
Anti-inflammation
Endophytic fungus
Mice
Millettia - chemistry
Millettia speciosa Champ
Penicillium - chemistry
Polysaccharide
Polysaccharides - chemistry
RAW 264.7 Cells
Structure
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Summary:An acid polysaccharide, named HP, was produced by endophytic Penicillium javanicum MSC-R1 isolated from southern medicine Millettia speciosa Champ. The molecular weight of HP was 37.8 kDa and consisted of Ara f, Galр, Glcр, Manр, and GlcрA with a molar ratio of 1.09: 3.47: 68.48: 16.59: 8.85. The glycosidic linkage of HP was proven to be →3, 4)-α–D-Glcр-(1→6)-α-D-Manр-(1→, →3, 4)-α-D-Glcр-(1→4)-α-D-Glcр-(1→, →3), →6)-α-D-Manр-(1→4)-α-D-Glcр-(1→, →3), β-D-Galр-(1→3)-α-D-Glcр-(1→, →4), →5)-α-L-Ara f –(1→3)-α-D-Glcр-(1→, →4), →6)-α-D-Manр-(1→4)-α-D-GlcAр-(1→ and →4)-α-D-GlcAр-(1→4)-α-D-Glcр-(1→, →3). Additionally, 250 μg/mL of HP possessed nontoxicity to RAW 264.7 cells and exhibited anti-inflammation activity. HP could significantly restrain the amount of tumor necrosis factor-α, interleukin-6 and NO release in RAW264.7, which property is possibly associated with its abundant glucosidic linkage. These results indicated that HP could be regarded as a ponderable ingredient for the health-beneficial functional foods. [Display omitted] •Endophytic fungi were selected by EPS yield and antioxidant activity of polysaccharide from M.speciosa.•Molecular weight and monosaccharide composition of HP were similar to polysaccharide extracted from the roots of M.speciosa.•HP exhibited a triple helical conformation with abundant glucosidic linkage.•HP showed significantly anti-inflammatory activity associated with structural characteristics.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2022.12.008