Chondroitin sulfate is not digested at all in the mouse small intestine but may suppress interleukin 6 expression induced by tumor necrosis factor-α

Salmon nasal cartilage proteoglycan (PG) was orally administered to mice. The PG digest was recovered from the small intestine, and its sugar chain size and unsaturated disaccharide content were examined. The elution position of the PG digest following Sepharose CL-4B chromatography was consistent w...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2023-01, Vol.642, p.185-191
Main Authors: Kudo, Kai, Kobayashi, Takashi, Kasai, Kosuke, Nozaka, Hiroyuki, Nakamura, Toshiya
Format: Article
Language:English
Subjects:
Animals
Chondroitin
Chondroitin sulfate
Chondroitin Sulfate Proteoglycans - metabolism
Chondroitin Sulfates - metabolism
Cytokines
Disaccharides
Humans
Inflammation
Interleukin-6
Mice
Proteoglycan
Proteoglycans - metabolism
Small intestine
Tumor Necrosis Factor-alpha
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Salmon nasal cartilage proteoglycan (PG) was orally administered to mice. The PG digest was recovered from the small intestine, and its sugar chain size and unsaturated disaccharide content were examined. The elution position of the PG digest following Sepharose CL-4B chromatography was consistent with that of actinase-digested PG prior to administration. The PG digest was incubated with chondroitinase ABC, which resulted in the elution pattern of the unsaturated disaccharides being identical to that of the degraded product of actinase-digested PG. The core protein of PG was digested in the mouse small intestine, but chondroitin sulfate, which is the sugar chain of PG, was not degraded at all. Then, the effects of chondroitin 4- and 6-sulfates on human colon cancer cells were examined. These chondroitin sulfates were found to suppress the expression of interleukin-6 induced by TNF-α. Overall, the chondroitin sulfate chain may act on the intestinal epithelium and suppress inflammation of the intestinal tract. •Chondroitin sulfate is not digested or degraded at all in the mouse small intestine.•Chondroitin sulfate suppresses IL6 expression in a small intestinal epithelial model.•Oral administration of proteoglycans may help ameliorate inflammatory bowel disease.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2022.12.051