Fabrication of antibacterial and biocompatible 3D printed Manuka-Gelatin based patch for wound healing applications

[Display omitted] •3D printing of Gelatin-based hydrogel with Manuka honey (MH-Gel);•The developed MH-Gel 3D patches have antimicrobial activity against Gram-positive and Gram-negative bacteria;•The 3D MH-Gelatin patches showed high cytocompatibility and the ability to promote angiogenesis. Developm...

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Bibliographic Details
Published in:International journal of pharmaceutics 2023-02, Vol.632, p.122541-122541, Article 122541
Main Authors: Brites, Ana, Ferreira, Marta, Bom, Sara, Grenho, Liliana, Claudio, Ricardo, Gomes, Pedro S., Fernandes, Maria H., Marto, Joana, Santos, Catarina
Format: Article
Language:English
Subjects:
3D Printing
Anti-Bacterial Agents - chemistry
Antibacterial
Cytocompatibility
Escherichia coli
Gelatin
Gelatin-based hydrogel
Honey - analysis
Humans
Hydrogels
Manuka Honey
Microbial Sensitivity Tests
Printing, Three-Dimensional
Staphylococcus aureus
Wound Healing
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Summary:[Display omitted] •3D printing of Gelatin-based hydrogel with Manuka honey (MH-Gel);•The developed MH-Gel 3D patches have antimicrobial activity against Gram-positive and Gram-negative bacteria;•The 3D MH-Gelatin patches showed high cytocompatibility and the ability to promote angiogenesis. Development of multifunctional 3D patches with appropriate antibacterial and biocompatible properties is needed to deal with wound care regeneration. Combining gelatin-based hydrogel with a well-known natural antibacterial honey (Manuka honey, MH) in a 3D patch can provide improved printability and at the same time provide favourable biological effects that may be useful in regenerative wound treatment. In this study, an antibacterial Manuka-Gelatin 3D patches was developed by an extrusion-based printing process, with controlled porosity, high shape fidelity, and structural stability. It was demonstrated the antibacterial activity of Manuka-Gelatin 3D patches against both gram-positive bacteria (S. epidermidis and S. aureus) and gram-negative (E. coli), common in wound infection. The 3D Manuka-Gelatin base patches demonstrated antibacterial activity, and moreover enhanced the proliferation of human dermal fibroblasts and human epidermal keratinocytes, and promotion of angiogenesis. Moreover, the ease of printing achieved by the addition of honey, coupled with the interesting biological response obtained, makes this 3D patch a good candidate for wound healing applications.
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2022.122541