Evaluation of specificity and sensitivity of IRT/IRT protocol in the cystic fibrosis newborn screening program: 6-year experience of three tertiary centers

We aimed to evaluate cutoff values of immunoreactive trypsinogen (IRT)/IRT and determine relationship between IRT values and clinical characteristics of children with cystic fibrosis (CF). This study is cross-sectional study. Data of children with positive newborn screening (NBS) between 2015 and 20...

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Bibliographic Details
Published in:	European journal of pediatrics 2023-03, Vol.182 (3), p.1067-1076
Main Authors:	Ramasli Gursoy, Tugba, Asfuroglu, Pelin, Sismanlar Eyuboglu, Tugba, Aslan, Ayse Tana, Yilmaz, Asli Imran, Unal, Gokcen, Kibar, Büsra Sultan, Pekcan, Sevgi, Hangul, Melih, Kose, Mehmet, Budakoglu, Isil Irem, Acican, Deniz
Format:	Article
Language:	eng
Subjects:	Child Cross-Sectional Studies Cystic Fibrosis - diagnosis Cystic Fibrosis Transmembrane Conductance Regulator - genetics Humans Infant, Newborn Medicine Medicine & Public Health Neonatal Screening - methods Pediatrics Sensitivity and Specificity Trypsinogen
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Summary:	We aimed to evaluate cutoff values of immunoreactive trypsinogen (IRT)/IRT and determine relationship between IRT values and clinical characteristics of children with cystic fibrosis (CF). This study is cross-sectional study. Data of children with positive newborn screening (NBS) between 2015 and 2021 were evaluated in three pediatric pulmonology centers. Age at admission, sex, gestational age, presence of history of meconium ileus, parental consanguinity, sibling with CF, and doll-like face appearance, first and second IRT values, sweat chloride test, fecal elastase, fecal fat, biochemistry results, and age at CF diagnosis were recorded. Sensitivity and specificity of IRT cutoff values were evaluated. Of 815 children with positive NBS, 58 (7.1%) children were diagnosed with CF. Median values of first and second IRT were 157.2 (103.7–247.6) and 113.0 (84.0–201.5) μg/L. IRT values used in current protocol, sensitivity was determined as 96.6%, specificity as 17.2% for first IRT, and 96.6% sensitivity, 20.5% specificity for second IRT. Positive predictive value (PPV) was determined as 7.1%. When cutoff value for first IRT was estimated as 116.7 μg/L, sensitivity was 69.0% and specificity was 69.6%, and when cutoff value was set to 88.7 μg/L for second IRT, sensitivity was 69.0% and specificity was 69.0%. Area under curve was 0.757 for first and 0.763 for second IRT ( p
ISSN:	1432-1076 1432-1076