Is losartan a promising agent for the treatment of type 1 diabetes-induced testicular germ cell apoptosis in rats?

Background Diabetes mellitus (DM) is common metabolic disease that poses a major risk to public health and fertility. Previous studies indicate that DM may cause male infertility by triggering oxidative stress and germ cell apoptosis in the testis. Due to the undesirable effects of known antidiabeti...

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Bibliographic Details
Published in:Molecular biology reports 2023-03, Vol.50 (3), p.2195-2205
Main Authors: Buhur, Aylin, Gürel, Çevik, Kuşçu, Gökçe Ceren, Yiğittürk, Gürkan, Oltulu, Fatih, Karabay Yavaşoğlu, Nefise Ülkü, Uysal, Ayşegül, Yavaşoğlu, Altuğ
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animals
Antidiabetics
Apoptosis
Bcl-2 protein
Biomedical and Life Sciences
Caspase-3
Diabetes
Diabetes mellitus (insulin dependent)
Diabetes Mellitus, Experimental - metabolism
Diabetes Mellitus, Type 1 - metabolism
DNA nucleotidylexotransferase
Drug dosages
Fertility
Germ Cells - metabolism
Histology
Infertility
Life Sciences
Losartan - pharmacology
Losartan - therapeutic use
Male
Metabolic disorders
Morphology
Original Article
Oxidative Stress
Public health
Rats
Streptozocin
Streptozocin - adverse effects
Testes
Testis - metabolism
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Summary:Background Diabetes mellitus (DM) is common metabolic disease that poses a major risk to public health and fertility. Previous studies indicate that DM may cause male infertility by triggering oxidative stress and germ cell apoptosis in the testis. Due to the undesirable effects of known antidiabetic drugs, scientists have begun to investigate the use of alternative drugs to control infertility complications observed in men. In this context, present study aimed to investigate the possible antiapoptotic effect of losartan against DM-induced testicular germ cell apoptosis. Methods and results Expreimental DM model was induced by intraperitoneal injection of streptozocin (STZ, 55 mg/kg) to 28 rats, which were then randomly assigned to 4 groups; 1 mL saline solution was given to DM + saline group by oral gavage, 5 mg/kg/day oral losartan was given to DM + low-dose losartan, 20 mg/kg/day oral losartan was given to DM + mid-dose losartan and, 80 mg/kg/day oral losartan was given to DM + high-dose losartan group for 4 weeks. Bax, Bcl-2 and cleaved-Caspase 3 immunoexpression, terminal-deoxynucleotidyl transferase dutp nick end labeling (TUNEL), Annexin-V and Real Time PCR analyses performed to evaluate antiapoptotic effects of losartan on diabetic rats’ testis. In addition, biochemical analyzes carried out to evaluate change in oxidative stress. Conclusion The results showed that losartan may have dose-related antiapoptotic effects on rats’ testis via decreasing oxidative stress.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-022-08172-9