Multispecies comparison of host responses to Fusarium circinatum challenge in tropical pines show consistency in resistance mechanisms

Fusarium circinatum poses a threat to both commercial and natural pine forests. Large variation in host resistance exists between species, with many economically important species being susceptible. Development of resistant genotypes could be expedited and optimised by investigating the molecular me...

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Published in:Plant, cell and environment cell and environment, 2023-05, Vol.46 (5), p.1705-1725
Main Authors: Visser, Erik A., Kampmann, Tamanique P., Wegrzyn, Jill L., Naidoo, Sanushka
Format: Article
Language:English
Subjects:
Biosynthesis
Coniferous forests
Flavonoids
Fusarium
Fusarium - physiology
Fusarium circinatum
Genetic resources
Genotype
Genotypes
host‐pathogen interaction
Jasmonic acid
Metabolism
Metabolites
Molecular modelling
Pine
Pine trees
Pinus
Pinus - genetics
Pinus greggii
Pinus maximinoi
Pinus oocarpa
Pinus patula
Pinus tecunumanii
Plant Diseases - genetics
Salicylic acid
Sulfur
Transcriptome - genetics
transcriptome assembly
Transcriptomes
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Summary:Fusarium circinatum poses a threat to both commercial and natural pine forests. Large variation in host resistance exists between species, with many economically important species being susceptible. Development of resistant genotypes could be expedited and optimised by investigating the molecular mechanisms underlying host resistance and susceptibility as well as increasing the available genetic resources. RNA‐seq data, from F. circinatum inoculated and mock‐inoculated ca. 6‐month‐old shoot tissue at 3‐ and 7‐days postinoculation, was generated for three commercially important tropical pines, Pinus oocarpa, Pinus maximinoi and Pinus greggii. De novo transcriptomes were assembled and used to investigate the NLR and PR gene content within available pine references. Host responses to F. circinatum challenge were investigated in P. oocarpa (resistant) and P. greggii (susceptible), in comparison to previously generated expression profiles from Pinus tecunumanii (resistant) and Pinus patula (susceptible). Expression results indicated crosstalk between induced salicylate, jasmonate and ethylene signalling is involved in host resistance and compromised in susceptible hosts. Additionally, higher constitutive expression of sulfur metabolism and flavonoid biosynthesis in resistant hosts suggest involvement of these metabolites in resistance. Summary statement F. circinatum resistant pines showed higher constitutive sulfur and flavonoid metabolism gene expression and susceptible hosts showed compromised induced responses. Counteracting these compromised pathways through hormone treatment or nutrient supplementation could improve pine resistance.
ISSN:0140-7791
1365-3040
DOI:10.1111/pce.14522