Highly gallol-substituted, rapidly self-crosslinkable, and robust chitosan hydrogel for 3D bioprinting

Although three-dimensional (3D) bioprinting is a promising technology for reconstructing artificial tissues and organs using bioink, there is a lack of a bioink that satisfies all requirements, including printability, gelation, mechanical properties, and cytocompatibility, Herein, a novel self-cross...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2023-02, Vol.227, p.493-504
Main Authors: Gwak, Min A., Lee, Su Jin, Lee, Dongjin, Park, Su A., Park, Won Ho
Format: Article
Language:English
Subjects:
3D bioprinting
Bioink
Bioprinting - methods
Chitosan
Conjugate
Gallic acid
Hydrogels - chemistry
Printing, Three-Dimensional
Rheology
Self-crosslinkable hydrogel
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Although three-dimensional (3D) bioprinting is a promising technology for reconstructing artificial tissues and organs using bioink, there is a lack of a bioink that satisfies all requirements, including printability, gelation, mechanical properties, and cytocompatibility, Herein, a novel self-crosslinkable bioink derived from chitosan (CS) and gallic acid (GA) is presented. 3D printed scaffolds with excellent shape fidelity are realized by systematically analyzing the self-crosslinking mechanism of hydrogel formation from CS-GA conjugates and by optimizing various parameters of the printing process. The CS-GA hydrogel forms rapidly in a physiological pH without any chemical crosslinking agent. In addition, the CS-GA hydrogel exhibited various physical and chemical intermolecular interactions, fast gelation rates, and excellent mechanical properties (>337 kPa). Moreover, the CS-GA hydrogel singificantly improves the cell viability (>92 %) and proliferation of the bioink. Therefore, the self-crosslinkable CS-GA bioink has great potential to overcome the limitations of conventional bioinks. •Novel self-crosslinkable chitosan (CS)-gallic acid (GA) conjugate bioink was designed.•Multiple crosslinking mechanisms of CS-GA hydrogel were systematically analyzed.•CS-GA bioink exhibited a rapid gelation and excellent mechanical properties.•3D-printed CS-GA bioink scaffolds exhibited good cytocompatibility and biodegradability.•CS-GA bioink has great potential for tissue engineering and regenerative medicine.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2022.12.124