PSMA2 knockdown impacts expression of proteins involved in immune and cellular stress responses in human lung cells

Proteasome subunit alpha type-2 (PSMA2) is a critical component of the 20S proteasome, which is the core particle of the 26S proteasome complex and is involved in cellular protein quality control by recognizing and recycling defective proteins. PSMA2 expression dysregulation has been detected in dif...

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Published in:Biochimica et biophysica acta. Molecular basis of disease 2023-02, Vol.1869 (2), p.166617-166617, Article 166617
Main Authors: Rashid, Mahamud-ur, Lorzadeh, Shahrokh, Gao, Ang, Ghavami, Saeid, Coombs, Kevin M.
Format: Article
Language:English
Subjects:
Autophagy, endoplasmic reticulum stress
Humans
Immune system
Lung - metabolism
Proteasome
Proteome - metabolism
PSMA2
Signal Transduction
Unfolded Protein Response
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Summary:Proteasome subunit alpha type-2 (PSMA2) is a critical component of the 20S proteasome, which is the core particle of the 26S proteasome complex and is involved in cellular protein quality control by recognizing and recycling defective proteins. PSMA2 expression dysregulation has been detected in different human diseases and viral infections. No study yet has reported PSMA2 knockdown (KD) effects on the cellular proteome. Methods: We used SOMAScan, an aptamer-based multiplexed technique, to measure >1300 human proteins to determine the impact of PSMA2 KD on A549 human lung epithelial cells. Results: PSMA2 KD resulted in significant dysregulation of 52 cellular proteins involved in different bio-functions, including cellular movement and development, cell death and survival, and cancer. The immune system and signal transduction were the most affected cellular functions. PSMA2 KD caused dysregulation of several signaling pathways involved in immune response, cytokine signaling, organismal growth and development, cellular stress and injury (including autophagy and unfolded protein response), and cancer responses. Conclusions: In summary, this study helps us better understand the importance of PSMA2 in different cellular functions, signaling pathways, and human diseases. •PSMA2, a critical component of the 20S and 26S proteasome, is involved in key cellular functions•SOMAscan analysis identified many lung cell proteins either up- or down-regulated by PSMA2 knockdown.•PSMA2 knockdown affected proteins involved in immune response, cytokine signaling, cellular stress and injury, and cancer responses.
ISSN:0925-4439
1879-260X
DOI:10.1016/j.bbadis.2022.166617