Proteome changes in autosomal recessive primary microcephaly

Background/aim : Autosomal recessive primary microcephaly (MCPH) is a rare and genetically heterogeneous group of disorders characterized by intellectual disability and microcephaly at birth, classically without further organ involvement. MCPH3 is caused by biallelic variants in the cyclin‐dependent...

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Bibliographic Details
Published in:Annals of human genetics 2023-03, Vol.87 (1-2), p.50-62
Main Authors: Zaqout, Sami, Mannaa, Atef, Klein, Oliver, Krajewski, Angelika, Klose, Joachim, Luise‐Becker, Lena, Elsabagh, Ahmed, Ferih, Khaled, Kraemer, Nadine, Ravindran, Ethiraj, Makridis, Konstantin, Kaindl, Angela M.
Format: Article
Language:English
Subjects:
Animals
Apoptosis
brain
Cdk5rap2
Cell cycle
Cell Cycle Proteins - genetics
Cytoskeleton
Electrophoretic mobility
Germ cells
Humans
Intellectual disabilities
MCPH
Mice
Microcephaly
Microcephaly - genetics
Microencephaly
Mutants
Mutation
Nerve Tissue Proteins - genetics
Neural stem cells
Phenotypes
proteome
Proteome - genetics
Proteomes
Proteomics
Substantia grisea
Synaptogenesis
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Summary:Background/aim : Autosomal recessive primary microcephaly (MCPH) is a rare and genetically heterogeneous group of disorders characterized by intellectual disability and microcephaly at birth, classically without further organ involvement. MCPH3 is caused by biallelic variants in the cyclin‐dependent kinase 5 regulatory subunit‐associated protein 2 gene CDK5RAP2. In the corresponding Cdk5rap2 mutant or Hertwig's anemia mouse model, congenital microcephaly as well as defects in the hematopoietic system, germ cells and eyes have been reported. The reduction in brain volume, particularly affecting gray matter, has been attributed mainly to disturbances in the proliferation and survival of early neuronal progenitors. In addition, defects in dendritic development and synaptogenesis exist that affect the excitation‐inhibition balance. Here, we studied proteomic changes in cerebral cortices of Cdk5rap2 mutant mice. Material and methods : We used large‐gel two‐dimensional gel (2‐DE) electrophoresis to separate cortical proteins. 2‐DE gels were visualized by a trained observer on a light box. Spot changes were considered with respect to presence/absence, quantitative variation and altered mobility. Result : We identified a reduction in more than 30 proteins that play a role in processes such as cell cytoskeleton dynamics, cell cycle progression, ciliary functions and apoptosis. These proteome changes in the MCPH3 model can be associated with various functional and morphological alterations of the developing brain. Conclusion : Our results shed light on potential protein candidates for the disease‐associated phenotype reported in MCPH3.
ISSN:0003-4800
1469-1809
DOI:10.1111/ahg.12489