Are intratumoral microbiota involved in the progression of intraductal papillary mucinous neoplasms of the pancreas?

Microbiota have been reported to influence the development of various gastrointestinal neoplasms through the mechanism of sustained inflammation; however, few data are available regarding their influence on intraductal papillary mucinous neoplasms. The aim of this study was to assess the association...

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Bibliographic Details
Published in:Surgery 2023-02, Vol.173 (2), p.503-510
Main Authors: Hozaka, Yuto, Oi, Hideyuki, Satake, Souichi, Uchino, Yoshinori, Goto, Yuichi, Idichi, Tetsuya, Tanoue, Kiyonori, Yamasaki, Yoichi, Kawasaki, Yota, Mataki, Yuko, Kurahara, Hiroshi, Nakajo, Akihiro, Higashi, Michiyo, Tanimoto, Akihide, Sugiura, Tsuyoshi, Ohtsuka, Takao
Format: Article
Language:English
Subjects:
Adenocarcinoma, Mucinous - pathology
Adenocarcinoma, Mucinous - surgery
Carcinoma, Pancreatic Ductal - pathology
Humans
Pancreas - surgery
Pancreatectomy
Pancreatic Intraductal Neoplasms - surgery
Pancreatic Neoplasms - pathology
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Summary:Microbiota have been reported to influence the development of various gastrointestinal neoplasms through the mechanism of sustained inflammation; however, few data are available regarding their influence on intraductal papillary mucinous neoplasms. The aim of this study was to assess the association between specific microbiota and the clinicopathologic characteristics of intraductal papillary mucinous neoplasms of the pancreas. DNA was extracted from formalin-fixed, paraffin-embedded samples of 30 patients who underwent pancreatectomy for intraductal papillary mucinous neoplasm, and polymerase chain reaction was used to create sequence libraries using the primer set for the V3 and V4 region of 16S recombinant DNA. Filtered sequence reads were then processed into operational taxonomic units with a 97% identity threshold and the relative abundance of bacteria compared between the 2 groups using operational taxonomic units. There was a trend toward fewer Firmicutes and more Proteobacteria and Fusobacteria in the relative abundance of main duct operational taxonomic units than in branch duct operational taxonomic units. The relative abundances of Bacteroidetes (P < .01) and Fusobacteria (P = .04) were significantly higher in invasive intraductal papillary mucinous neoplasms than in noninvasive intraductal papillary mucinous neoplasms. The relative abundance of the intestinal type was significantly lower in Firmicutes than the relative abundance of the nonintestinal type (P = .04). Notably, main duct operational taxonomic units with the intestinal subtype were affected by increased proportions of Proteobacteria and Fusobacteria, and Fusobacteria were abundant in the intestinal type of invasive main duct operational taxonomic units. Intratumoral microbiota may be involved in the progression of operational taxonomic units.
ISSN:0039-6060
1532-7361
DOI:10.1016/j.surg.2022.10.003