Semisynthesis, in silico study and in vitro antibacterial evaluation of fucosterol derivatives

[Display omitted] •Thirteen fucosterol derivatives were prepared.•Antibacterial activity was evaluated against Gram(+) and Gram(-) bacterial strains.•The most active compounds were investigated by molecular docking. Thirteen fucosterol derivatives were prepared by structural modification at the hydr...

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Published in:Steroids 2023-01, Vol.189, p.109137-109137, Article 109137
Main Authors: da Silva, Francisco E.F., Ávila, Fábio do N., Pereira, Nicaely M.O., de Freitas, Maria D., Pessoa, Otília D.L., da Fonseca, Aluísio M., da Costa, José G.M., Santiago, Gilvandete M.P.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Antibacterial activity
Bacteria
Fucosterol derivatives
Lobophora variegata
Microbial Sensitivity Tests
Molecular docking
Molecular Docking Simulation
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Summary:[Display omitted] •Thirteen fucosterol derivatives were prepared.•Antibacterial activity was evaluated against Gram(+) and Gram(-) bacterial strains.•The most active compounds were investigated by molecular docking. Thirteen fucosterol derivatives were prepared by structural modification at the hydroxyl group in C-3 and catalytic hydrogenation at the carbon–carbon double bond in C-5(6) and C-24(28). The structures of all compounds were established based on their spectral data (IR, MS, and NMR). Fucosterol (1) and its derivatives (2-12, and a mixture of 13a and 13b) were evaluated for their in vitro antibacterial activity against Klebsiella pneumoniae (ATCC 10031), Escherichia coli (ATCC 10536), Pseudomonas aeruginosa (ATCC 15442), Streptococcus mutans (ATCC 0046) and Staphylococcus aureus using the microdilution method. Among them, 1, 8, 9, 10, and a mixture of 13a and 13b exhibited the best antibacterial activity. The derivative 7 was inactive against all bacterial strains evaluated (MIC ≥ 2.327 mM). In addition, the investigation of binding interactions of more active compounds (1, 8, 9, 10, and mixture of 13a and 13b) to appropriate proteins was performed using molecular docking. This paper registers for the first time the in silico studies on the antibacterial activity of compounds 1, 8, 9, 10, and mixture of 13a/13b, and the spectral data of compounds 4, 6, and 7.
ISSN:0039-128X
1878-5867
DOI:10.1016/j.steroids.2022.109137