Five NUCLEAR FACTOR-Y subunit B genes in rapeseed (Brassica napus) promote flowering and root elongation in Arabidopsis

Main conclusion Heterologous expression of BnNF-YB2 , BnNF-YB3 , BnNF-YB4 , BnNF-YB5 , or BnNF-YB6 from rapeseed promotes the floral process and also affects root development in Arabidopsis. The transcriptional regulator NUCLEAR FACTOR-Y (NF-Y) is a heterotrimeric complex composed of NF-YA, NF-YB, a...

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Bibliographic Details
Published in:Planta 2022-12, Vol.256 (6), p.115-115, Article 115
Main Authors: Fu, Ruixin, Wang, Ji, Zhou, Mengjia, Ren, Xuyang, Hua, Jianyang, Liang, Mingxiang
Format: Article
Language:English
Subjects:
Agriculture
Arabidopsis
Biomedical and Life Sciences
Brassica
Brassica napus
Developmental stages
Ecology
Elongation
Flowering
Forestry
Genes
Life Sciences
Nucleotide sequence
Original Article
Phylogeny
Plant Sciences
Proteins
Rape plants
Rapeseed
Root development
Simulators
Yeasts
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Summary:Main conclusion Heterologous expression of BnNF-YB2 , BnNF-YB3 , BnNF-YB4 , BnNF-YB5 , or BnNF-YB6 from rapeseed promotes the floral process and also affects root development in Arabidopsis. The transcriptional regulator NUCLEAR FACTOR-Y (NF-Y) is a heterotrimeric complex composed of NF-YA, NF-YB, and NF-YC proteins and is ubiquitous in yeast, animal, and plant systems. In this study, we found that five NF-YB proteins from rapeseed ( Brassica napus ), including BnNF-YB2, BnNF-YB3, BnNF-YB4, BnNF-YB5, and BnNF-YB6 (BnNF-YB2/3/4/5/6), all function in photoperiodic flowering and root elongation. Sequence alignment and phylogenetic analysis showed that BnNF-YB2/3 and BnNF-YB4/5/6 were clustered with Arabidopsis AtNF-YB2 and AtNF-YB3, respectively, implying that these NF-YBs are evolutionarily and functionally conserved. In support of this hypothesis, the heterologous expression of individual BnNF-YB2 , 3 , 4 , 5 , or 6 in Arabidopsis promoted early flowering under a long-day photoperiod. Further analysis suggested that BnNF-YB 2/3/4/5/6 elevated the expression of key downstream flowering time genes including CO , FT , LFY and SOC1 . Promoter-GUS fusion analysis showed that the five BnNF-YBs were expressed in a variety of tissues at various developmental stages and GFP fusion analysis revealed that all BnNF-YBs were localized to the nucleus. In addition, we demonstrated that the heterologous expression of individual BnNF-YB2 / 3 / 4 / 5 / 6 in Arabidopsis promoted root elongation and increased the number of root tips formed under both normal and treatment with simulators of abiotic stress conditions.
ISSN:0032-0935
1432-2048
DOI:10.1007/s00425-022-04030-x